Wang Qinjing, Huang Zhijie, Guo Jiming, Chen Weixing, Wang Min, Ming Yue, Liu Hongyu, Huang Mingshu, Huang Yisheng, Tang Zhengming, Jia Bo
Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou 510261, China.
Biomolecules. 2025 Jul 22;15(8):1055. doi: 10.3390/biom15081055.
Plant-derived vesicle-like nanoparticles (PDVLNs) are bioactive nanovesicles secreted by plant cells, emerging as a novel therapeutic tool for tissue repair and regeneration due to their low immunogenicity, intrinsic bioactivity, and potential as drug delivery carriers. This review examines PDVLNs' biogenesis mechanisms, isolation techniques, and compositional diversity, emphasizing their roles in promoting essential regenerative processes-cell proliferation, differentiation, migration, immune modulation, and angiogenesis. We explore their therapeutic applications across multiple tissue types, including skin, bone, neural, liver, gastrointestinal, cardiovascular, and dental tissues, using both natural and engineered PDVLNs in various disease models. Compared to mammalian exosomes, PDVLNs offer advantages such as reduced immune rejection and ethical concerns, enhancing their sustainability and appeal for regenerative medicine. However, challenges in clinical translation, including scalability, standardization, and safety remain. This paper consolidates current knowledge on PDVLNs, highlighting their versatility and providing insights into engineering strategies to optimize efficacy, ultimately outlining future research directions to advance their clinical potential. Plant vesicle-like nanoparticles (PDVLNs) may become a new avenue for the treatment of tissue injury, promoting tissue repair and regeneration through their intrinsic bioactivity or as drug delivery carriers. In addition, PDVLNs can be engineered and modified to achieve better results.
植物来源的囊泡样纳米颗粒（PDVLNs）是植物细胞分泌的生物活性纳米囊泡，由于其低免疫原性、内在生物活性以及作为药物递送载体的潜力，正成为一种用于组织修复和再生的新型治疗工具。本综述探讨了PDVLNs的生物发生机制、分离技术和组成多样性，强调了它们在促进细胞增殖、分化、迁移、免疫调节和血管生成等重要再生过程中的作用。我们研究了它们在多种组织类型中的治疗应用，包括皮肤、骨骼、神经、肝脏、胃肠道、心血管和牙科组织，在各种疾病模型中使用天然和工程化的PDVLNs。与哺乳动物外泌体相比，PDVLNs具有减少免疫排斥和伦理问题等优势，增强了它们在再生医学中的可持续性和吸引力。然而，临床转化方面仍存在挑战，包括可扩展性、标准化和安全性。本文整合了关于PDVLNs的现有知识，突出了它们的多功能性，并提供了优化疗效的工程策略见解，最终概述了推进其临床潜力的未来研究方向。植物囊泡样纳米颗粒（PDVLNs）可能成为治疗组织损伤的新途径，通过其内在生物活性或作为药物递送载体促进组织修复和再生。此外，PDVLNs可以进行工程设计和修饰以取得更好的效果。
