Gao Zujie, Xu Jinlong, Zhao Hengxin, Zheng Xiaobing, Lyu Zijian, Liu Qiwei, Chen Hao, Zhang Yu, Li He-Ping, Li Yongjian
Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Department of Engineering Physics, Tsinghua University, Beijing 100084, China.
Biomolecules. 2025 Jul 25;15(8):1077. doi: 10.3390/biom15081077.
Although cold atmospheric plasma (CAP) has shown promise in facilitating wound repair due to its non-thermal and non-invasive properties, its dynamic effects on cellular response and metabolic regulation remain poorly characterized, and the mechanism is still unclear. In this study, we developed a microfluidic experimental system that integrates a CAP treatment module with multiparametric in situ sensing capabilities, along with precise environmental control of temperature, humidity, and CO concentration. A stratified microfluidic chip was engineered to co-culture HaCaT keratinocytes and HSF fibroblasts. CAP treatment was applied within this platform, and the dynamic processes of cell migration, proliferation, and multiple metabolic markers were simultaneously monitored. The experimental results show that the system can not only achieve real-time observation in the healing process under plasma intervention, but also find that the healing process is closely related to the concentration of NO. In addition, the study also found that keratin KRT14, which is thought to be closely related to wound healing, decreased significantly in the process of plasma-induced healing. The platform provides high-resolution experimental tools to elucidate the biological effects of CAP and has the potential for parameter optimization, material evaluation, and personalized therapeutic development to advance plasma research and clinical translational applications.
尽管冷大气等离子体（CAP）因其非热和非侵入性特性在促进伤口修复方面显示出前景，但其对细胞反应和代谢调节的动态影响仍缺乏充分表征，机制也尚不清楚。在本研究中，我们开发了一种微流控实验系统，该系统集成了具有多参数原位传感能力的CAP处理模块，以及对温度、湿度和CO浓度的精确环境控制。设计了一种分层微流控芯片，用于共培养HaCaT角质形成细胞和HSF成纤维细胞。在该平台内进行CAP处理，并同时监测细胞迁移、增殖和多种代谢标志物的动态过程。实验结果表明，该系统不仅可以在等离子体干预下的愈合过程中实现实时观察，还发现愈合过程与NO浓度密切相关。此外，该研究还发现，被认为与伤口愈合密切相关的角蛋白KRT14在等离子体诱导愈合过程中显著降低。该平台提供了高分辨率实验工具来阐明CAP的生物学效应，并具有参数优化、材料评估和个性化治疗开发的潜力，以推进等离子体研究和临床转化应用。
