Cerda-Celis Andrea, Vidal Mabel, Goldstein Merari, Santillán-Araneda Maria Jesús, Rivera Alexis, Vargas Daniela, Jerez Gabriel, Vallejos-Vidal Eva, Reyes-Cerpa Sebastian, Reyes-López Felipe E
Fish Health and Integrative Physiogenomics Research Team, Centro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Avenida Libertador Bernardo OHiggins 3363, Edificio de Investigación Eduardo Morales, Santiago 9170002, Chile.
Departamento de Ingeniería Informática y Ciencias de la Computación, Universidad de Concepción, Edmundo Larenas 219, Concepción 4070409, Chile.
Animals (Basel). 2025 Aug 12;15(16):2369. doi: 10.3390/ani15162369.
Chile is the second-largest producer of Atlantic salmon (), a key industry facing challenges such as infestations by , which cause significant economic losses. However, there are no studies exploring how environmental variables in marine culture centers affect salmon's immune response, considering their poikilothermic characteristics. This study analyzed the effect of the seasonal temperature on the skin transcriptome of Atlantic salmon infested with in fjord-based farms in the Aysén Region during autumn and spring. Two different centers were analyzed (autumn: Farm-A; spring: Farm-S) to ensure the same age of the fish sampled in each season. RNA sequencing (RNA-seq) and functional network analysis revealed notable seasonal transcriptomic differences. In autumn, 253 differentially expressed genes (DEGs) were found, primarily associated with stress response and negative regulation of T-cell proliferation, suggesting an impaired response against the ectoparasite. In spring, 103 DEGs were detected, associated with protein synthesis and the activation of immune mechanisms, including complement activation, granulocyte chemotaxis, and antigen presentation. This is the first study to evaluate the transcriptomic response of healthy skin in -infested salmon under farming conditions, highlighting the importance of considering seasonal variations for the development of more effective management strategies in the aquaculture industry.
智利是大西洋鲑鱼的第二大生产国，而大西洋鲑鱼养殖业作为关键产业面临着诸如海虱侵扰等挑战，这些侵扰会造成重大经济损失。然而，鉴于鲑鱼的变温特性，尚无研究探讨海洋养殖中心的环境变量如何影响鲑鱼的免疫反应。本研究分析了秋季和春季期间，季节性温度对阿伊森地区峡湾养殖场中感染海虱的大西洋鲑鱼皮肤转录组的影响。分析了两个不同的养殖中心（秋季：养殖场A；春季：养殖场S），以确保每个季节所采样的鱼年龄相同。RNA测序（RNA-seq）和功能网络分析揭示了显著的季节性转录组差异。在秋季，发现了253个差异表达基因（DEG），主要与应激反应和T细胞增殖的负调控相关，这表明对体外寄生虫的反应受损。在春季，检测到103个DEG，与蛋白质合成和免疫机制的激活相关，包括补体激活、粒细胞趋化性和抗原呈递。这是第一项评估养殖条件下感染海虱的鲑鱼健康皮肤转录组反应的研究，突出了考虑季节变化对于水产养殖业制定更有效管理策略的重要性。
