Thongsima Thitiporn, Pakdeesanaeha Thitida, Techarungchaikul Sirichai, Jintana Ratree, Towanabutr Norraset, Santiviparat Sawita, Bhanpattanakul Sudchaya, Udomthanaisit Larindhorn, Tharasanit Theerawat
Obstetrics, Gynaecology and Reproduction Unit, Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
The College of Veterinary Specialties of Thailand, Nonthaburi 11000, Thailand.
Animals (Basel). 2025 Aug 13;15(16):2379. doi: 10.3390/ani15162379.
Thwaites (LST) has emerged as a potential supplement for enhancing male reproductive performance. This study evaluated the effects of long-term oral supplementation with LST on hematological parameters, semen characteristics, ultrasonographic measurements of the prostate gland and testes, and the cryopreservation potential of canine sperm. Six healthy male dogs received oral LST supplementation at a dosage of 10 mg/kg body weight once daily for 7 days (short-term). After a three-month washout period to ensure full physiological recovery, the same dogs underwent a long-term supplementation protocol (60 days). In the short-term trial, no clinically significant changes were observed in hematological or serum biochemical parameters, including complete blood count, alanine aminotransferase, creatinine, blood urea nitrogen, total protein, and albumin; all parameters were within normal reference ranges. Serum testosterone levels and semen characteristics were also unaffected ( > 0.05). During the long-term treatment, blood profiles and testosterone levels remained stable. Although prostatic and testicular volumes increased slightly, the changes were not statistically significant ( > 0.05). A significant increase in semen volume was observed ( < 0.05), while other semen parameters showed no significant differences. Notably, post-thaw sperm motility significantly improved at both 15 min and 4 h after thawing, and sperm viability was significantly enhanced at 4 h post-thaw ( < 0.05), suggesting a potential protective effect of LST during cryopreservation. These findings indicate that LST supplementation is physiologically safe and may improve canine sperm quality during freezing and thawing, supporting its potential application in reproductive health management.
斯韦茨（LST）已成为一种潜在的用于提高雄性生殖性能的补充剂。本研究评估了长期口服LST对血液学参数、精液特征、前列腺和睾丸的超声测量以及犬精子冷冻保存潜力的影响。六只健康雄性犬每天接受一次口服LST补充剂，剂量为10毫克/千克体重，持续7天（短期）。经过三个月的洗脱期以确保完全生理恢复后，相同的犬只接受了长期补充方案（60天）。在短期试验中，血液学或血清生化参数未见临床显著变化，包括全血细胞计数、丙氨酸转氨酶、肌酐、血尿素氮、总蛋白和白蛋白；所有参数均在正常参考范围内。血清睾酮水平和精液特征也未受影响（>0.05）。在长期治疗期间，血液指标和睾酮水平保持稳定。虽然前列腺和睾丸体积略有增加，但变化无统计学意义（>0.05）。观察到精液体积显著增加（<0.05），而其他精液参数无显著差异。值得注意的是，解冻后15分钟和4小时的解冻后精子活力均显著提高，解冻后4小时精子存活率显著增强（<0.05），表明LST在冷冻保存期间具有潜在的保护作用。这些发现表明，补充LST在生理上是安全的，并且可能在冷冻和解冻过程中改善犬精子质量，支持其在生殖健康管理中的潜在应用。
