Reichl D, Forte T M, Hong J L, Rudra D N, Pflug J
J Lipid Res. 1985 Dec;26(12):1399-411.
The concentration of cholesterol, apolipoproteins A-I, B, and E has been determined in lymphedema fluid from nine patients with chronic primary lymphedema. The concentrations were: 38.14 +/- 21.06 mg/dl for cholesterol, 15.6 +/- 6.17 mg/dl for apolipoprotein A-I, 7.5 +/- 2.8 mg/dl for apolipoprotein B, and 1.87 +/- 0.50 mg/dl for apolipoprotein E. These values represent 23%, 12%, 6%, and 38% of plasma concentrations, respectively. The ratio of esterified to unesterified cholesterol in lymphedema fluid was 1.46 +/- 0.45. Lipoproteins of lymphedema fluid were fractionated according to particle size by gradient gel electrophoresis and by exclusion chromatography. Gradient gel electrophoresis showed that a majority of high density lipoproteins (HDL) of lymphedema fluid were larger than ferritin (mol wt 440,000) and smaller than low density lipoproteins (LDL); several discrete subpopulations could be seen with the large HDL region. Fractionation by exclusion chromatography showed that more than 25% of apolipoprotein A-I and all of apolipoprotein E in lymphedema fluid was associated with particles larger than plasma HDL2. Apolipoprotein A-I also eluted in fractions that contained particles the size of or smaller than albumin. Isolation of lipoproteins by sequential ultracentrifugation showed that less than 25% of lymphedema fluid cholesterol was associated with apolipoprotein B. The majority of apolipoprotein A-containing lipoproteins of lymphedema fluid were less dense than those in plasma. Ultracentrifugally separated fractions of lipoproteins were examined by electron microscopy. The fraction d less than 1.019 g/ml contained little material, while fraction d 1.019-1.063 g/ml contained two types of particles: round particles 17-26 nm in diameter and square-packing particles 13-17 nm on a side. Fractions d 1.063-1.085 g/ml had extensive arrays of square-packing particles 13-14 nm in size. Fractions d 1.085-1.11 g/ml and fractions d 1.11-1.21 g/ml contained round HDL, 12-13 nm diameter and 10 nm diameter, respectively. Discoidal particles were observed infrequently.
已测定9例慢性原发性淋巴水肿患者淋巴水肿液中胆固醇、载脂蛋白A-I、B和E的浓度。具体浓度如下：胆固醇为38.14±21.06mg/dl，载脂蛋白A-I为15.6±6.17mg/dl，载脂蛋白B为7.5±2.8mg/dl，载脂蛋白E为1.87±0.50mg/dl。这些值分别相当于血浆浓度的23%、12%、6%和38%。淋巴水肿液中酯化胆固醇与未酯化胆固醇的比例为1.46±0.45。通过梯度凝胶电泳和排阻色谱法根据颗粒大小对淋巴水肿液中的脂蛋白进行分级分离。梯度凝胶电泳显示，淋巴水肿液中的大多数高密度脂蛋白（HDL）大于铁蛋白（分子量440,000）且小于低密度脂蛋白（LDL）；在大HDL区域可见几个离散的亚群。排阻色谱法分级分离显示，淋巴水肿液中超过25%的载脂蛋白A-I和所有载脂蛋白E与大于血浆HDL2大小的颗粒相关。载脂蛋白A-I也在含有与白蛋白大小相同或更小的颗粒的级分中洗脱。通过连续超速离心法分离脂蛋白显示，淋巴水肿液中不到25%的胆固醇与载脂蛋白B相关。淋巴水肿液中大多数含载脂蛋白A的脂蛋白比血浆中的密度低。通过电子显微镜检查超速离心分离的脂蛋白级分。密度小于1.019g/ml的级分几乎不含物质，而密度为1.019 - 1.063g/ml的级分含有两种类型的颗粒：直径为17 - 26nm的圆形颗粒和边长为13 - 17nm的方形堆积颗粒。密度为1.063 - 1.085g/ml的级分有大量尺寸为13 - 14nm的方形堆积颗粒阵列。密度为1.085 - 1.11g/ml的级分和密度为1.11 - 1.21g/ml的级分分别含有直径为12 - 13nm和10nm的圆形HDL。很少观察到盘状颗粒。
