Rudra D N, Myant N B, Pflug J J, Reichl D
Atherosclerosis. 1984 Dec;53(3):297-308. doi: 10.1016/0021-9150(84)90130-8.
The lipoproteins of peripheral lymph and plasma from normal human subjects were separated according to their density by sequential ultracentrifugation and according to their size by gradient gel electrophoresis and gel exclusion chromatography. High density lipoproteins (HDL) carried a higher proportion of the total cholesterol in lymph than in plasma. Within the HDL fraction, the less dense and more lipid-rich component (HDL2) carried a higher proportion of the total HDL cholesterol in lymph than in plasma. Gradient gel electrophoresis showed (1) a higher proportion of large to small HDL particles in lymph than in plasma and (2) the presence of at least three populations of apo A-I-containing lipoproteins with Stokes diameters larger than the Stokes diameter of HDL2. Separation by gel exclusion chromatography showed that the proportion of large HDL particles with a high cholester: apo A-I ratio was greater in lymph than in plasma. In view of the sieving effect of the blood capillaries, which favours the passage across the capillary walls of smaller vs larger particles, we suggest that the higher ratio of large to small HDL particles in lymph than in plasma is due to the conversion of small to large HDL in the interstitial fluid by incorporation of cholesterol and other lipids from extravascular cells into the smaller particles.
通过连续超速离心根据密度以及通过梯度凝胶电泳和凝胶排阻色谱根据大小对正常人体受试者外周淋巴和血浆中的脂蛋白进行分离。高密度脂蛋白（HDL）在淋巴中携带的总胆固醇比例高于血浆。在HDL组分中，密度较低且脂质含量较高的成分（HDL2）在淋巴中携带的HDL总胆固醇比例高于血浆。梯度凝胶电泳显示：（1）淋巴中大小HDL颗粒的比例高于血浆；（2）存在至少三种斯托克斯直径大于HDL2斯托克斯直径的含载脂蛋白A-I的脂蛋白群体。凝胶排阻色谱分离显示，胆固醇与载脂蛋白A-I比例高的大HDL颗粒在淋巴中的比例高于血浆。鉴于毛细血管的筛分作用有利于较小颗粒而非较大颗粒穿过毛细血管壁，我们认为淋巴中大小HDL颗粒的比例高于血浆是由于间质液中小HDL通过从血管外细胞摄取胆固醇和其他脂质转化为大HDL所致。
