Su Chaoqun, Tan Chenxi, Zhao Liangjie, Liu Jiahui, Guo Xusheng, Yao Gaoyou, Zhang Weizhao, Yang Tiezhu
School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China.
Fishery Biological Engineering Technology Research Center of Henan Province, Xinyang 464000, China.
Genes (Basel). 2025 Aug 19;16(8):977. doi: 10.3390/genes16080977.
: , an endemic fish in the Huaihe River basin, is a newly described species with limited molecular genetic research, hindering understanding of its evolutionary status, population structure, and genetic diversity. This study aimed to characterize its complete mitochondrial genome, clarify its phylogenetic position within , and assess its population genetic diversity. : We obtained the complete mitogenome of (sourced from the Zhugan River) through sequencing, followed by detailed annotation of this genomic sequence. We analyzed its genomic structure, nucleotide composition, codon usage, and base asymmetry. Selection pressure on 13 protein-coding genes (PCGs) was evaluated using Ka/Ks ratios. Phylogenetic trees were generated by means of Bayesian inference (BI) and maximum likelihood (ML), using a dataset composed of 13 protein-coding genes (PCGs) from 37 species. Population genetic diversity was assessed using the gene. : The mitogenome is a 16,512 bp circular molecule encoding 37 genes and one control region, with a conserved structure typical of . It has high A + T content (55.74%) with A-preference and C-enrichment. All PCGs undergo purifying selection (Ka/Ks < 1). Phylogenetic analyses revealed is closest to (100% support), with divided into three clades. The gene analysis showed low diversity (Hd = 0.656, π = 0.00171) and neutral evolution. : This study fills the mitogenome data gap for , clarifies its evolutionary characteristics and phylogenetic position, and provides a basis for conservation genetics of Huaihe endemic fishes and molecular evolution research on Amblycipitidae.
作为淮河盆地的一种特有鱼类，是一个新描述的物种，其分子遗传学研究有限，这阻碍了对其进化地位、种群结构和遗传多样性的理解。本研究旨在表征其完整的线粒体基因组，阐明其在[物种名]内的系统发育位置，并评估其种群遗传多样性。我们通过测序获得了[物种名]（源自竹竿河）的完整线粒体基因组，随后对该基因组序列进行了详细注释。我们分析了其基因组结构、核苷酸组成、密码子使用情况和碱基不对称性。使用Ka/Ks比率评估13个蛋白质编码基因（PCG）的选择压力。通过贝叶斯推断（BI）和最大似然法（ML）构建系统发育树，使用由37个物种的13个蛋白质编码基因（PCG）组成的数据集。使用[基因名]基因评估种群遗传多样性。线粒体基因组是一个16,512 bp的环状分子，编码37个基因和一个控制区，具有[物种名]典型的保守结构。它具有较高的A + T含量（55.74%），偏好A且富含C。所有PCG都经历纯化选择（Ka/Ks < 1）。系统发育分析表明[物种名]与[另一物种名]最接近（支持率100%），[物种名]分为三个分支。[基因名]基因分析显示多样性较低（Hd = 0.656，π = 0.00171）且呈中性进化。本研究填补了[物种名]线粒体基因组数据空白，阐明了其进化特征和系统发育位置，并为淮河特有鱼类的保护遗传学和钝头鮠科分子进化研究提供了依据。
