The Complete Mitochondrial Genome of Red Costate Tiger Moth ( [Cramer, 1777]), and Phylogenetic Analyses of the Subfamily Arctiinae.

BACKGROUND/OBJECTIVES: , class Insecta, order Lepidoptera, superfamily Noctuoidea, family Erebidae, and subfamily Arctiinae, is a polytrophic agricultural pest. However, there are still many sequences missing for Arctiinae from mitochondrial whole-genome sequences.

METHODS

In this study, we determined and analyzed the complete mitochondrial genome sequence of . Furthermore, based on the sequencing results, we used the Bayesian inference, maximum likelihood, and maximum reduction methods to analyze the phylogenies of 18 species of the Hypophora subfamily.

RESULTS

The mitochondrial genome was found to be a circular double-stranded DNA with a length of 15,380 bp and included 13 protein-coding genes (PCG), 22 tRNA genes, 2 rRNA genes, and one control region. With the exception of , all the tRNA genes could form conventional clover structures. There were 23 intergenic spacer regions with lengths of 1-52 bp and six gene overlaps with lengths of 1-8 bp. The control region was located between and genes and comprised 303 bp and an AT content of 74.25%.

CONCLUSIONS

The results showed that is closely related to . Our results suggest that Syntomini is phylogenetically distinct from Arctiini and may warrant separate tribal status within Arctiinae. This study is dedicated to researching the mitochondrial genome and phylogenetic relationships of , providing a molecular basis for its classification.