Gao Tianyu, Zhang Chenlu, Hu Xueqiang, Wang Tianqi, Lyu Zhitang, Sun Lei
College of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding 071002, China.
Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, China.
Microorganisms. 2025 Aug 1;13(8):1798. doi: 10.3390/microorganisms13081798.
In this study, a highly cadmium (II)-resistant bacterium strain, C10-4, identified as , was isolated from a sediment sample collected from Baiyangdian Lake, China. The minimum inhibitory concentration (MIC) of Cd(II) for strain C10-4 was 1600 mg/L. Factors such as the contact time, pH, Cd(II) concentration, and biomass dosage affected the adsorption of Cd(II) by strain C10-4. The adsorption process fit well to the Langmuir adsorption isotherm model and the pseudo-second-order kinetics model, based on the Cd(II) adsorption data obtained from the cells of strain C10-4. This suggests that Cd(II) is adsorbed by strain C10-4 cells via a single-layer homogeneous chemical adsorption process. According to the Langmuir model, the maximum biosorption capacity was 3.31 mg/g for fresh-strain C10-4 biomass. Cd(II) was shown to adhere to the bacterial cell wall through SEM-EDS analysis. FTIR spectroscopy further indicated that the main functional sites for the binding of Cd(II) ions on the cell surface of strain C10-4 were functional groups such as N-H, -OH, -CH-, C=O, C-O, P=O, sulfate, and phosphate. After the inoculation of strain C10-4 into Cd(II)-contaminated soils, there was a significant reduction ( < 0.01) in the exchangeable fraction of Cd and an increase ( < 0.01) in the sum of the reducible, oxidizable, and residual fractions of Cd. The results show that C10-4 has good potential for use in the remediation of Cd(II)-contaminated soils.
在本研究中，从中国白洋淀采集的沉积物样本中分离出一株对镉（II）具有高度抗性的细菌菌株C10 - 4，鉴定为[此处原文缺失具体菌种信息]。菌株C10 - 4对Cd（II）的最小抑菌浓度（MIC）为1600 mg/L。接触时间、pH值、Cd（II）浓度和生物量剂量等因素影响菌株C10 - 4对Cd（II）的吸附。基于从菌株C10 - 4细胞获得的Cd（II）吸附数据，吸附过程很好地符合朗缪尔吸附等温线模型和准二级动力学模型。这表明Cd（II）通过单层均匀化学吸附过程被菌株C10 - 4细胞吸附。根据朗缪尔模型，新鲜菌株C10 - 4生物量对Cd（II）的最大生物吸附容量为3.31 mg/g。通过扫描电子显微镜 - 能谱分析（SEM - EDS）表明Cd（II）附着在细菌细胞壁上。傅里叶变换红外光谱（FTIR）进一步表明，菌株C10 - 4细胞表面上结合Cd（II）离子的主要功能位点是诸如N - H、 - OH、 - CH -、C = O、C - O、P = O、硫酸盐和磷酸盐等官能团。将菌株C10 - 4接种到受Cd（II）污染的土壤中后，Cd的可交换部分显著降低（<0.01），而Cd的可还原、可氧化和残留部分的总和增加（<0.01）。结果表明，C10 - 4在修复受Cd（II）污染的土壤方面具有良好的应用潜力。
