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精油作为控制从……分离出的多种真菌的抗真菌替代品:第一部分。

Essential Oils as an Antifungal Alternative for the Control of Various Species of Fungi Isolated from : Part I.

作者信息

Ruiz Medina Maritza D, Ruales Jenny

机构信息

Departamento de Ciencias de Alimentos y Biotecnología (DECAB), Escuela Politécnica Nacional (EPN), Quito 170143, Ecuador.

出版信息

Microorganisms. 2025 Aug 5;13(8):1827. doi: 10.3390/microorganisms13081827.

Abstract

This study evaluated the antifungal potential of essential oils (EOs): oregano (), rosemary (), clove (), thyme (), cinnamon (), and basil (). These oils were tested against fungi isolated from banana peels (). The fungi tested were identified through macroscopic and microscopic analyses and DNA sequencing, after being isolated in potato dextrose agar (PDA) medium modified with 0.05% chloramphenicol. Subsequently, the antifungal properties of the tested essential oils were evaluated at concentrations of 200, 400, 600, 800, and 1000 ppm prepared in a 0.05% Tween 80 solution. Cinnamon EOs showed the highest antifungal activity, significantly inhibiting the growth of pathogens at a concentration of 400 ppm. Other EOs showed moderate effects at higher concentrations: rosemary inhibited fungal growth at 600 ppm, oregano at 800 ppm, and clove at 1000 ppm. These findings highlight the potential of EOs as eco-friendly alternatives to synthetic fungicides, contributing to the development of sustainable agricultural practices and the post-harvest management of bananas. It is recommended to conduct future research to assess the economic viability and practical impacts of large-scale applications.

摘要

本研究评估了香精油(EOs)的抗真菌潜力:牛至、迷迭香、丁香、百里香、肉桂和罗勒。这些香精油针对从香蕉皮中分离出的真菌进行了测试。在添加0.05%氯霉素的马铃薯葡萄糖琼脂(PDA)培养基中分离后,通过宏观和微观分析以及DNA测序对测试的真菌进行鉴定。随后,在0.05%吐温80溶液中制备浓度为200、400、600、800和1000 ppm的测试香精油,评估其抗真菌特性。肉桂香精油表现出最高的抗真菌活性,在浓度为400 ppm时能显著抑制病原体的生长。其他香精油在较高浓度下表现出中等效果:迷迭香在600 ppm时抑制真菌生长,牛至在800 ppm时抑制,丁香在1000 ppm时抑制。这些发现凸显了香精油作为合成杀菌剂的环保替代品的潜力,有助于可持续农业实践的发展以及香蕉的采后管理。建议开展未来研究以评估大规模应用的经济可行性和实际影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4adf/12388783/9b3ca33ce0f2/microorganisms-13-01827-g001.jpg

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