Bu Sihan, Nel Nikita H, Beavers Alyssa W, Sugino Kameron Y, Alaimo Katherine, Biernbaum John A, Comstock Sarah S
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824, USA.
Department of Nutrition and Food Science, Wayne State University, Detroit, MI 48201, USA.
Microorganisms. 2025 Aug 13;13(8):1885. doi: 10.3390/microorganisms13081885.
The aim of this study was to determine the impact of two root media and three vermicompost amendments on the root zone bacterial communities and harvest mass of lettuce grown in a greenhouse container garden model system. Lettuce seeds were planted in seven root media/amendment conditions. Lettuce was later harvested, and root media DNA was extracted for 16S rRNA sequencing to determine the composition of, as well as the alpha and beta diversity of, the bacterial communities. Fresh weight, dry weight, and percentage dry weight of lettuce were calculated under each treatment. Results indicate that the peat-lite growth media without any additions had the lowest rhizosphere bacterial alpha diversity compared to the other six growth media. Bacterial communities from containers with peat-lite media were significantly different than those from containers with compost-based media as measured by beta diversity. Moreover, the compost-based medium with vermicompost condition tended to result in a higher percentage dry weight lettuce than lettuce grown under the peat-lite condition. The peat-lite treatment condition had the numerically lowest dry weight (%) and bacterial diversity. Addition of vermicompost amendments had varying impacts on bacterial diversity, bacterial community composition, and harvest mass. Overall, this experiment establishes a protocol which can be applied for further understanding of the impact of root media type and vermicompost amendments on rhizosphere bacterial diversity and harvest mass.
本研究的目的是确定两种根系培养基质和三种蚯蚓粪改良剂对温室容器花园模型系统中种植的生菜根区细菌群落和收获质量的影响。将生菜种子种植在七种根系培养基质/改良剂条件下。随后收获生菜，并提取根系培养基质DNA进行16S rRNA测序，以确定细菌群落的组成以及其α和β多样性。计算每种处理下生菜的鲜重、干重和干重百分比。结果表明，与其他六种生长培养基质相比，未添加任何物质的泥炭蛭石生长培养基质的根际细菌α多样性最低。通过β多样性测量，泥炭蛭石培养基质容器中的细菌群落与基于堆肥的培养基质容器中的细菌群落显著不同。此外，添加蚯蚓粪的基于堆肥的培养基质往往会使生菜的干重百分比高于在泥炭蛭石条件下生长的生菜。泥炭蛭石处理条件下的干重（%）和细菌多样性在数值上最低。添加蚯蚓粪改良剂对细菌多样性、细菌群落组成和收获质量有不同的影响。总体而言，本实验建立了一个方案，可用于进一步了解根系培养基质类型和蚯蚓粪改良剂对根际细菌多样性和收获质量的影响。
