Silva Leandro Augusto Mariano, Pereira Natália, Menezes Mareliza Possa de, Rodrigues Romário Alves, Reis Milena Souza, Oliveira Giordano Eugenio, Santos Hugo Leandro Dos, Pizauro Lucas José Luduverio, Ludgero Ana Karen de Mendonça, Duarte Christiane Eliza Motta, Oliveira Leandro Licursi de, Bragança Caio Roberto Soares, Cardozo Marita Vedovelli
Department of Pathology Reproduction and One Health, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal 14884-900, Brazil.
Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal 14884-900, Brazil.
Microorganisms. 2025 Aug 15;13(8):1901. doi: 10.3390/microorganisms13081901.
The BOL lectin, a 34 kDa protein with a hemagglutination titer of 64 hemagglutination units (HU), was extracted from cauliflower ( spp. L.), purified by affinity and ion exchange chromatography, and confirmed, in this study, by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The antibiofilm activity of BOL was evaluated at two concentrations (0.1 mg/mL and 1.0 mg/mL) against bacterial strains of importance to human health ( ATCC 10876, ATCC 25922, ATCC 29213, and ATCC 12403). In addition to a biofilm formation assay, a pre-formed biofilm assay was conducted, with biofilm structure analyzed by Scanning Electron Microscopy (SEM). The antimicrobial potential of BOL was also investigated using the Minimum Inhibitory Concentration (MIC) assay in 96-well microplates. Among the tested bacterial strains, BOL exhibited activity against at 1.0 mg/mL, interfering with both biofilm formation and disrupting pre-formed biofilms, which may be explained by a possible interaction between BOL and the components present in the biofilm matrix. However, no antibiofilm activity was observed against , , or , possibly due to differences in the composition of their biofilm matrices. Furthermore, BOL showed no detectable bactericidal or bacteriostatic activity in the antimicrobial assays. In conclusion, BOL lectin, at the tested concentrations, does not exhibit direct antimicrobial activity but effectively disrupts the extracellular matrix in ATCC 29213.
BOL凝集素是一种34 kDa的蛋白质，血凝效价为64个血凝单位（HU），从花椰菜（ 种， 属）中提取，通过亲和色谱和离子交换色谱进行纯化，并在本研究中通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行了确认。在两种浓度（0.1 mg/mL和1.0 mg/mL）下评估了BOL对人类健康重要的细菌菌株（ATCC 10876、ATCC 25922、ATCC 29213和ATCC 12403）的抗生物膜活性。除了生物膜形成试验外，还进行了预形成生物膜试验，通过扫描电子显微镜（SEM）分析生物膜结构。还使用96孔微孔板中的最低抑菌浓度（MIC）试验研究了BOL的抗菌潜力。在测试的细菌菌株中，BOL在1.0 mg/mL时对 表现出活性，干扰生物膜形成并破坏预形成的生物膜，这可能是由于BOL与生物膜基质中存在的成分之间可能的相互作用所致。然而，未观察到对 、 或 的抗生物膜活性，可能是由于它们生物膜基质组成的差异。此外，在抗菌试验中BOL未显示出可检测到的杀菌或抑菌活性。总之，在所测试的浓度下，BOL凝集素不表现出直接的抗菌活性，但能有效破坏ATCC 29213中的细胞外基质。
