Xu Zhanhong, Yang Wanxia, Shang Xulan, Fu Xiangxiang, Sun Caowen, Fang Shengzuo
State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2025 Aug 11;14(16):2495. doi: 10.3390/plants14162495.
To reveal the effects of genotype-herbivore interactions on leaf quality, foliar variations in phytochemicals, morphoanatomy, and herbivory damage ratio were investigated in a (Batalin) Iljinsk. (Juglandaceae) germplasm resources bank. Results showed less herbivory damage in genotypes with a higher leaf thickness, but more herbivory damage in genotypes with a higher leaf stomatal density. Herbivory damage ratios were significantly correlated with the contents of leaf secondary metabolites, whereas the response of secondary metabolites to insect attack was type-specific and varied between intact leaves and damaged leaves. Based on key indicators of leaf quality (contents of triterpenoids, flavonoids, polyphenols, pterocaryoside A, pterocaryoside B, and cyclocaric acid B), the investigated genotypes were divided into three distinct groups by integrating TOPSIS and cluster analysis, while four genotypes with slight insect damage demonstrated the prioritization for future applications. Our findings lay a foundation for further selection of its superior varieties with both insect resistance and high leaf quality.
为揭示基因型与食草动物相互作用对叶片质量的影响，在一个胡桃（胡桃科）种质资源库中，对植物化学物质、形态解剖学和食草损伤率的叶片变化进行了研究。结果表明，叶片厚度较高的基因型食草损伤较少，而叶片气孔密度较高的基因型食草损伤较多。食草损伤率与叶片次生代谢物含量显著相关，而次生代谢物对昆虫攻击的反应具有类型特异性，且在完整叶片和受损叶片之间有所不同。基于叶片质量的关键指标（三萜类、黄酮类、多酚类、胡桃苷A、胡桃苷B和环胡桃酸B的含量），通过集成TOPSIS法和聚类分析，将所研究的基因型分为三个不同的组，同时，四种食草损伤轻微的基因型显示出未来应用的优先性。我们的研究结果为进一步选育具有抗虫性和高叶片质量的优良品种奠定了基础。
