Liang Sijia, Wang Qiongqiong, Han Qin, Zhang Xinmin, Liu Yiyuan, Chen Miaosheng, Zhang Chengcai, Wang Zhaoyang, Li Junxiao, Yu Di, Zhan Hao, Zhang Yubin, Xu Zhongping
Academy of Industry Innovation and Development, Huanghuai University, Zhumadian 463000, China.
College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.
Plants (Basel). 2025 Aug 16;14(16):2549. doi: 10.3390/plants14162549.
Turcz. (), a perennial herb in the Santalaceae family, exhibits potent antibacterial and anti-inflammatory properties. Transcriptome sequencing was performed on one- and two-year-old plants across seedling, flowering, and fruiting stages (all sampled from the same location) using the illumina NovaSeq 6000 platform. A total of 58,706 unigenes were identified, including 1656 transcription factors (TFs). Further analysis classified these TFs into seven functional categories, enabling the reconstruction of a representative TF regulatory network. Differential expression analysis revealed that the number of differentially expressed genes (DEGs) ranged from 2000 to 5000 during different developmental stages in first-year plants, while varying between 1000 and 2000 in second-year plants. Comparative analysis of DEGs between one- and two-year-old plants showed that they were primarily associated with sesquiterpene, triterpene, and terpene skeleton biosynthesis, as well as other metabolic pathways. Additionally, analysis of key genes involved in flavonoid biosynthesis-the major bioactive compounds in -revealed their predominant accumulation during the first year of growth. This study provides valuable insights into the developmental biology of and establishes a foundation for future research on flavonoid biosynthesis pathway genes and their therapeutic applications.
檀香科的多年生草本植物土沉香（Turcz.）具有强大的抗菌和抗炎特性。使用Illumina NovaSeq 6000平台，对同一地点采集的一年生和两年生植株在幼苗期、花期和果期进行了转录组测序。共鉴定出58,706个单基因，其中包括1656个转录因子（TFs）。进一步分析将这些TFs分为七个功能类别，从而重建了一个具有代表性的TF调控网络。差异表达分析显示，一年生植株在不同发育阶段差异表达基因（DEGs）的数量在2000至5000之间，而两年生植株则在1000至2000之间。一年生和两年生植株DEGs的比较分析表明，它们主要与倍半萜、三萜和萜类骨架生物合成以及其他代谢途径相关。此外，对参与黄酮类生物合成（主要生物活性化合物）的关键基因的分析表明，它们在生长的第一年积累最多。本研究为土沉香的发育生物学提供了有价值的见解，并为未来黄酮类生物合成途径基因及其治疗应用的研究奠定了基础。
