Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, School of Pharmacy, Anhui Medical University, Hefei, China.
Pharm Biol. 2023 Sep 7;61(1):1446-1453. doi: 10.1080/13880209.2023.2253841.
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still spreading rapidly. Relevant research based on the antiviral effects of Turcz (Santalaceae) was not found.
To investigate the antiviral and anti-inflammatory effects of extracts of .
To investigate the anti-entry and replication effect of the ethanol extract of (drug concentration 80, 160, 320, 640, 960 μg/mL) against the SARS-CoV-2. Remdesivir (20.74 μM) was used as positive control, and Vero cells were used as host cells to detect the expression level of nucleocapsid protein (NP) in the virus by real-time quantitative polymerase chain reaction (RT-PCR) and Western blotting. RAW264.7 cells were used as an anti-inflammatory experimental model under lipopolysaccharide (LPS) induction, and the expression levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA).
The ethanol extract of significantly inhibited the replication (half maximal effective concentration, EC: 259.3 μg/mL) and entry (EC: 359.1 μg/mL) of SARS-CoV-2 into Vero cells, and significantly reduced the levels of IL-6 and TNF-α produced by LPS-stimulated RAW264.7 cells. Petroleum ether (EC: 163.6 μg/mL), ethyl acetate (EC: 22.92 μg/mL) and -butanol (EC: 56.8 μg/mL) extracts showed weak inhibition of SARS-CoV-2 replication in Vero cells, and reduced the levels of IL-6 and TNF-α produced by LPS-stimulated RAW264.7 cells.
can be a potential candidate to fight SARS-CoV-2, and is becoming a traditional Chinese medicine candidate for treating COVID-19.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）仍在迅速传播。尚未发现基于 Turcz（檀香科）抗病毒作用的相关研究。
研究 提取物的抗病毒和抗炎作用。
研究了 80、160、320、640、960μg/ml 浓度乙醇提取物对 SARS-CoV-2 的进入和复制的抑制作用。以瑞德西韦（20.74μM）为阳性对照，以 Vero 细胞为宿主细胞，通过实时定量聚合酶链反应（RT-PCR）和 Western blot 检测病毒核衣壳蛋白（NP）的表达水平。用脂多糖（LPS）诱导 RAW264.7 细胞作为抗炎实验模型，通过酶联免疫吸附试验（ELISA）检测肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的表达水平。
乙醇提取物显著抑制 SARS-CoV-2 进入和进入 Vero 细胞的复制（半数有效浓度，EC：259.3μg/ml），并显著降低 LPS 刺激 RAW264.7 细胞产生的 IL-6 和 TNF-α水平。石油醚（EC：163.6μg/ml）、乙酸乙酯（EC：22.92μg/ml）和 -丁醇（EC：56.8μg/ml）提取物对 Vero 细胞中 SARS-CoV-2 的复制有较弱的抑制作用，并降低 LPS 刺激 RAW264.7 细胞产生的 IL-6 和 TNF-α水平。
可作为抗击 SARS-CoV-2 的潜在候选药物，有望成为治疗 COVID-19 的中药候选药物。
