Scharf Sebastian, Romero-Parra Javier, Winterhalter Peter, Torres-Benítez Alfredo, Gök Recep, Simirgiotis Mario J
Institute of Food Chemistry, Technische Universitat Braunschweig, Schleinitzstrase 20, 38106 Braunschweig, Germany.
Departamento de Química Orgánica y Fisicoquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 6640022, Chile.
Plants (Basel). 2025 Aug 17;14(16):2555. doi: 10.3390/plants14162555.
In recent years, numerous studies have emerged on the biological activities of endemic berries from the Valdivian Forest and their potential for therapeutic use. However, some species appear to have been relatively neglected. The objective of this study was to conduct, for the first time, a phytochemical composition analysis of a hydroalcoholic extract of Ruiz & Pav. and to evaluate its potential as an antioxidant and as an enzyme inhibitor in relation to chronic non-communicable diseases. The berries were collected in the Saval Park in Valdivia and were subsequently extracted via sonication in ethanol/water. UHPLC-DAD, HPLC-APCI (+)-MS, and UHPLC-ESI (+)-TOF-MS analysis allowed for the identification of several carotenoid ester species. According to UHPLC-DAD, the sum of carotenoids yielded was 983.4 ± 26.3 mg/kg DW, while the concentration of the phenolic compounds was 9.33 ± 0.01 mg GAE/g dry fruit. The extract exhibited antioxidant properties by scavenging DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azinobis-(3-ethylbenzothioazolin-6-sulfonic acid)) radicals, and ferric reducing antioxidant power (FRAP). It also demonstrated cholinesterase enzyme inhibitor capacities (AChE and BuChE-IC: 6.904 ± 0.42 and 18.38 ± 0.48 µg/mL, respectively). Docking calculations were additionally performed for a selection of compounds in the berries. The data obtained suggest that the hydroalcoholic extract of possesses significant potential as a natural antioxidant and for the inhibition of enzymes, making it a promising candidate for the development of phytotherapeutic and nutraceutical products, especially as a supplement against chronic diseases.
近年来，关于瓦尔迪维亚森林特有浆果的生物活性及其治疗用途潜力的研究大量涌现。然而，一些物种似乎相对受到忽视。本研究的目的是首次对Ruiz & Pav.的水醇提取物进行植物化学成分分析，并评估其作为抗氧化剂和慢性非传染性疾病相关酶抑制剂的潜力。浆果在瓦尔迪维亚的萨瓦尔公园采集，随后通过乙醇/水超声提取。超高效液相色谱-二极管阵列检测（UHPLC-DAD）、高效液相色谱-大气压化学电离（正离子模式）质谱（HPLC-APCI(+)-MS）和超高效液相色谱-电喷雾电离（正离子模式）飞行时间质谱（UHPLC-ESI(+)-TOF-MS）分析鉴定出了几种类胡萝卜素酯类物质。根据UHPLC-DAD分析，类胡萝卜素总量为983.4±26.3毫克/千克干重，而酚类化合物浓度为9.33±0.01毫克没食子酸当量/克干果。该提取物通过清除2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)（ABTS）自由基以及具有铁还原抗氧化能力（FRAP）表现出抗氧化特性。它还表现出胆碱酯酶抑制能力（乙酰胆碱酯酶和丁酰胆碱酯酶的半数抑制浓度分别为6.904±0.42和18.38±0.48微克/毫升）。此外，还对浆果中的部分化合物进行了对接计算。所获得的数据表明，Ruiz & Pav.的水醇提取物具有作为天然抗氧化剂和酶抑制剂的巨大潜力，使其成为植物治疗和营养保健品开发的有前景候选物，特别是作为对抗慢性病的补充剂。
