Anti- Compounds of Hance Branch.

Hance (Viburnaceae), the Korean elderberry, is widely used in herbal medicine and in the food industry. It is known to have various pharmacological effects, including antitumor, antioxidant, anti-inflammatory, and antimicrobial activities. During our search for anti- compounds from natural resources, the methanol extract of the branch significantly inhibited the growth of . Three phenolic and four lignan compounds were isolated from the methylene chloride fraction that had shown the most potent anti- activity among the hexane, methylene chloride, ethyl acetate, butanol, and water fractions. The chemical structures were identified to be three phenolics of sylvopinol (), dihydroconiferyl alcohol (), and (7,8)-guaiacylglycerol () and four lignans of boehmenan (), (7,8)-guaiacylglycerol -coniferyl ether () and lawsonicin () with a new lignan, (7,8)-sambucanol (), the structure of which was established by H- and C-NMR, and HRESI-MS, as well as quantum chemical electronic circular dichroism (ECD) calculations. Among the isolates, compounds and exhibited significant anti- activity against strains 51 and 26695. Compound displayed more potent antibacterial activity with MIC values of 3.13 and 6.25 μM, and MIC values of 28.5 and 56.8 μM against the two strains, respectively. Their inhibitory activities were higher than those of a positive control, quercetin. Furthermore, these two compounds showed moderate urease inhibitory activity. A molecular docking simulation revealed the high binding ability of and to the active site of urease. These results will provide further insights into the design of more potent natural products for eradicating .