Davoudi Pahnekolayi Mahboubeh, Parchianloo Zahra, Babouyehdarabi Majid, Ghasemi Meysam
Department of Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien (TU Wien), 1040 Vienna, Austria.
Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan M9PX+FX7, Iran.
Plants (Basel). 2025 Aug 21;14(16):2606. doi: 10.3390/plants14162606.
is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due to high horticultural consumption, applying the plant tissue culture technique as a major tool for healthy and massive-scale production of plants is not unexpected. However, the response of in vitro plantlets to various plant tissue culture ingredients is not well understood to tender an efficient applied protocol for qualitative and quantitative in vitro propagation. In this regard, the main objective of this study is to investigate the influence of several abiotic in vitro variants including six plant tissue culture media formulations (McCown's Woody Plant Medium (WPM), Murashige and Skoog (MS), Van der Salm (VS), Schenk and Hildebrant (SH), Driver Kuniyuki Walnut (DKW), and Gamburg B5 (B5)) in combination with four concentrations (0, 1.5, 3, 4 mgL) of two types of cytokinins (6-Benzyaminopurine (BAP) and Kinetin (Kin)) simultaneously. Notably, it is perceived that DKW culture medium containing 1.5 mgL BAP and 0.1 mgL NAA is the best treatment for both in vitro morphological and flowering properties.
是珍贵的本土玫瑰砧木之一，在植物生产者中享有很高声誉，具有与美容价值和次生代谢产物生产相关的潜在园艺和药理特性。由于园艺消费量大，将植物组织培养技术作为健康、大规模生产植物的主要工具也就不足为奇了。然而，离体苗对各种植物组织培养成分的反应尚未得到充分了解，难以提供一个用于定性和定量离体繁殖的有效应用方案。在这方面，本研究的主要目的是同时研究几种非生物离体变量的影响，包括六种植物组织培养基配方（麦考恩木本植物培养基（WPM）、Murashige和Skoog（MS）、范德萨尔姆（VS）、申克和希尔德布兰特（SH）、德赖弗·国行核桃（DKW）和甘伯格B5（B5））与两种细胞分裂素（6-苄氨基嘌呤（BAP）和激动素（Kin））的四种浓度（0、1.5、3、4 mg/L）的组合。值得注意的是，可以看出含有1.5 mg/L BAP和0.1 mg/L NAA的DKW培养基对离体形态和开花特性都是最佳处理。
