Institute of Plant Genetics, Leibniz Universität Hannover, Hannover, Germany.
Institute of Agricultural Genetics, Hanoi, Vietnam.
Plant Cell Rep. 2017 Sep;36(9):1493-1505. doi: 10.1007/s00299-017-2170-8. Epub 2017 Jun 24.
We analysed the capacity to regenerate adventitious shoots in 96 rose genotypes and found 88 SNP markers associated with QTLs, some of which are derived from candidate genes for shoot regeneration. In an association panel of 96 rose genotypes previously analysed for petal colour, we conducted a genome-wide association study on the capacity of leaf petioles for direct shoot regeneration. Shoot regeneration rate and shoot ratio (number of shoots/total number of explants) were used as phenotypic descriptors for regeneration capacity. Two independent experiments were carried out with six replicates of ten explants each. We found significant variation between the genotypes ranging from 0.88 to 88.33% for the regeneration rate and from 0.008 to 1.2 for the shoot ratio, which exceeded the rates reported so far. Furthermore, we found 88 SNP markers associated with either the shoot regeneration rate or the shoot ratio. In this association analysis, we found 12 SNP markers from ESTs (expressed sequence tags) matching known candidate genes that are involved in shoot morphogenesis. The best markers explained more than 51% of the variance in the shoot regeneration rate and more than 0.65 of the variance in the shoot regeneration ratio between the homozygote marker classes. The genes underlying some of the best markers such as a GT-transcription factor or an LRR receptor-like protein kinase are novel candidate genes putatively involved in the observed phenotypic differences. The associated markers were mapped to the closely related genome of Fragaria vesca and revealed many distinct clusters, which also comprised the known candidate genes that functioned in the organogenesis of plant shoots. However, the validation of candidate genes and their functional relationship to shoot regeneration require further analysis in independent rose populations and functional analyses.
我们分析了 96 个玫瑰基因型再生不定芽的能力，发现了 88 个与 QTL 相关的 SNP 标记，其中一些标记来自于与芽再生相关的候选基因。在之前分析花瓣颜色的 96 个玫瑰基因型的关联面板中，我们对叶片叶柄直接芽再生能力进行了全基因组关联研究。芽再生率和芽比（芽数/外植体总数）被用作再生能力的表型描述符。我们进行了两个独立的实验，每个实验有 6 个重复，每个重复有 10 个外植体。我们发现基因型之间存在显著差异，再生率范围为 0.88%至 88.33%，芽比范围为 0.008 至 1.2，超过了迄今为止报道的比率。此外，我们发现了 88 个与芽再生率或芽比相关的 SNP 标记。在这项关联分析中，我们从 EST（表达序列标签）中找到了 12 个与已知候选基因匹配的 SNP 标记，这些候选基因参与芽形态发生。最好的标记解释了超过 51%的芽再生率和超过 0.65的芽再生比率之间的方差，在纯合标记类之间。一些最好的标记（如 GT 转录因子或 LRR 受体样蛋白激酶）所涉及的基因是潜在的候选基因，它们可能参与了观察到的表型差异。相关标记被映射到亲缘关系密切的 Fragaria vesca 基因组上，揭示了许多不同的簇，其中还包括在植物芽器官发生中起作用的已知候选基因。然而，候选基因的验证及其与芽再生的功能关系需要在独立的玫瑰群体和功能分析中进一步分析。
