Freitas Tatiana Pádua Tavares de, Teixeira Bernardo Rodrigues, Machado Eduarda de Oliveira Silva Lima, Pinto Isaac Leandro Lira, Oliveira Laís da Silva de, Varella Karina, Santos Huarrisson Azevedo, Santos Fernando de Oliveira, Tiepolo Liliani Marilia, Massard Carlos Luiz, Peckle Maristela
Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro-UFRRJ, Seropédica 23890-000, RJ, Brazil.
Laboratory of Biology and Parasitology of Reservoir Wild Mammals (LABPMR), Oswaldo Cruz Institute-FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.
Pathogens. 2025 Jul 31;14(8):756. doi: 10.3390/pathogens14080756.
Small rodents are known hosts of various pathogens, including , but until now, in Brazil, only has been described in these animals. In this study, liver samples and blood smears were obtained from 289 rodents belonging to 14 Cricetidae and two Muridae species that had been captured in municipalities of the states of Paraná and Rio de Janeiro. Smears were stained with Giemsa, and gametocytes were detected via microscopy in 10.72% ( = 31/289) of samples, with these individuals representing three rodent species. Significant morphometric differences were observed in gametocyte measurements in rodents. Using conventional PCR, spp. 18S rDNA fragments were amplified in 24.91% ( = 72/289) of samples, with those individuals representing seven rodent species. Phylogenetic analyses clustered 41 sequences from this study into a subclade with other sequences from small mammals in Brazil, identifying four distinct haplotypes, and, for the first time, a relationship between haplotype and gametocyte length was observed. Based on phylogenetic analysis, this study reinforces the trophic relationship between rodents and reptiles as a possible link in the transmission cycle in South America. Furthermore, our findings expand knowledge on spp. hosts, describing and as new host species and identifying two novel circulating haplotypes in rodents from Paraná State, southern Brazil.
小型啮齿动物是多种病原体的已知宿主，包括……，但截至目前，在巴西，这些动物中仅描述了……。在本研究中，从巴拉那州和里约热内卢州各市捕获的属于14种仓鼠科和2种鼠科的289只啮齿动物身上采集了肝脏样本和血液涂片。涂片用吉姆萨染色，通过显微镜检查在10.72%（n = 31/289）的样本中检测到配子体，这些个体代表三种啮齿动物物种。在……啮齿动物的配子体测量中观察到显著的形态学差异。使用常规PCR，在24.91%（n = 72/289）的样本中扩增出……属18S rDNA片段，这些个体代表七种啮齿动物物种。系统发育分析将本研究中的41个序列与巴西小型哺乳动物的其他序列聚类到一个亚分支中，确定了四个不同的单倍型，并且首次观察到……单倍型与配子体长度之间的关系。基于系统发育分析，本研究强化了啮齿动物与爬行动物之间的营养关系，认为这可能是南美洲……传播循环中的一个环节。此外，我们的研究结果扩展了关于……属宿主的知识，将……和……描述为新的宿主物种，并在巴西南部巴拉那州的啮齿动物中鉴定出两种新的循环单倍型。
