Sandhu Jatinderpal K, Jones Donald J L, Maxwell Colleen B, Gwinnell Charlotte L, Williams Geraldine, Cao Thong Huy, Quinn Paulene A, Ng Leong L
Leicester van Geest MultiOMICs Facility, University of Leicester, Leicester, UK.
Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.
Rapid Commun Mass Spectrom. 2025 Dec 15;39(23):e10125. doi: 10.1002/rcm.10125.
Molecular imprinting has emerged as a promising strategy to create custom imprints for precision recognition of proteins. This study proposes using dopamine polymers as a novel approach to enhance the retrieval of proteins from human plasma. Dopamine polymers possess adhesive properties due to their ability to form hydrophobic interactions, π-π, hydrogen bonding and van der Waals forces with various substrates; in this study, we have leveraged these adhesive properties to capture and retrieve proteins from complex biological samples.
We imprinted proteins derived from mouse heart lysate and evaluated the performance of the resulting molecularly imprinted polymer for retrieval of the human protein cardiovascular disease plasma samples.
Our results demonstrated the retrieval of troponin T, fatty acid-binding protein, creatine kinase, lactate dehydrogenase, and myosin-binding protein C. This novel application of dopamine polymers in protein enrichment and analysis facilitates the discovery of novel biomarkers from complex matrices, such as plasma, and promotes deeper insights into complex biological processes.
This method, characterized by high specificity and stability, offers a new approach for the detection of low abundant proteins and provides a scientific basis for the future development of new diagnostic tools and personalized medical strategies.
分子印迹已成为一种有前景的策略，可用于创建定制印记以精确识别蛋白质。本研究提出使用多巴胺聚合物作为一种新方法来增强从人血浆中检索蛋白质的能力。多巴胺聚合物具有粘附特性，因为它们能够与各种底物形成疏水相互作用、π-π相互作用、氢键和范德华力；在本研究中，我们利用这些粘附特性从复杂的生物样品中捕获和检索蛋白质。
我们对从小鼠心脏裂解物中提取的蛋白质进行印迹，并评估所得分子印迹聚合物用于检索人蛋白质心血管疾病血浆样本的性能。
我们的结果表明可以检索到肌钙蛋白T、脂肪酸结合蛋白、肌酸激酶、乳酸脱氢酶和肌球蛋白结合蛋白C。多巴胺聚合物在蛋白质富集和分析中的这种新应用有助于从血浆等复杂基质中发现新型生物标志物，并促进对复杂生物过程的更深入了解。
该方法具有高特异性和稳定性，为低丰度蛋白质的检测提供了一种新方法，并为新型诊断工具和个性化医疗策略的未来发展提供了科学依据。
