Center for Supramolecular Chemical Biology, State Key Laboratory of Supramolecular Structure and Materials, School of Life Sciences, Jilin University, Changchun, 130012, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Angew Chem Int Ed Engl. 2023 May 2;62(19):e202213938. doi: 10.1002/anie.202213938. Epub 2023 Apr 3.
Phospholipids, as fundamental building blocks of the cell membrane, play important roles for molecule transportation, cell recognition, etc. However, due to the structural diversity and amphipathic nature, there are few methods for the specific recognition of lipids as compared to other biomolecules such as proteins and glycans. Herein, we developed a molecular imprinting strategy for controllable imprinting toward the polar head of phospholipid exposed on the surface of cellular membranes for recognition. Phosphatidylserine, as unique lipid on the outer membrane leaflet of exosome and also hallmark for cell apoptosis, was imprinted with the developed method. The phosphatidylserine imprinted materials showed high efficiency and specific targeting capability not only for apoptotic cell imaging but also for the isolation of exosomes. Collectively, the synthesized molecularly imprinted materials have great potential for selective plasma membrane recognition for targeted drug delivery and biomarker discovery.
磷脂作为细胞膜的基本组成部分，对于分子运输、细胞识别等具有重要作用。然而，与蛋白质和聚糖等其他生物分子相比，由于其结构多样性和两亲性，针对脂质的特异性识别方法较少。在此，我们开发了一种分子印迹策略，用于对细胞膜表面暴露的磷脂的极性头进行可控印迹，以实现识别。磷脂酰丝氨酸是外体膜小叶上独特的脂质，也是细胞凋亡的标志，用所开发的方法对其进行印迹。所合成的印迹材料不仅对凋亡细胞成像具有高效和特异性靶向能力，而且对 exosome 的分离也具有高效和特异性靶向能力。总之，所合成的分子印迹材料对于靶向药物递送和生物标志物发现的选择性质膜识别具有很大的潜力。
