Ishibashi T, Ichihara K, Abiko Y
Jpn Circ J. 1985 Oct;49(10):1090-8. doi: 10.1253/jcj.49.1090.
The isolated rat heart was perfused by Langendorff's technique with either an oxygenated (95% O2 and 5% CO2) or an anoxic (95% N2 and 5% CO2) solution. Anoxia increased coronary flow and effluent adenosine concentration, but the time course of these changes was discordant; coronary flow increased transiently (the maximum increase in flow was observed within 1-2 min after the onset of anoxia), whereas the effluent adenosine concentration increased as a function of the time during anoxia. The concentration of effluent adenosine reached 3 microM 10 min after anoxia. The tissue (myocardial) adenosine content changed in parallel with the effluent adenosine concentration. An infusion of adenosine increased coronary flow dose-dependently, and the maximum increase was obtained when 0.27 microM of adenosine was infused. Effluent adenosine concentration was 0.05-0.3 microM in both anoxia and adenosine infusion experiments at the time when the maximum increase in coronary flow occurred. An increase in concentrations of adenosine above 0.05-0.03 microM in the effluent was not accompanied by an increase in coronary flow. These results raise a question about the role of adenosine in regulating coronary circulation during anoxic perfusion, though there is a possibility of vasodilatory function of adenosine in early period of anoxic perfusion.
采用Langendorff技术，用含氧（95% O₂和5% CO₂）或缺氧（95% N₂和5% CO₂）溶液灌注离体大鼠心脏。缺氧增加了冠脉流量和流出液中腺苷浓度，但这些变化的时间进程不一致；冠脉流量短暂增加（缺氧开始后1 - 2分钟内观察到流量最大增加），而流出液中腺苷浓度随缺氧时间而增加。缺氧10分钟后，流出液中腺苷浓度达到3微摩尔/升。组织（心肌）腺苷含量与流出液中腺苷浓度平行变化。输注腺苷剂量依赖性地增加冠脉流量，输注0.27微摩尔/升腺苷时获得最大增加。在冠脉流量最大增加时，缺氧和腺苷输注实验中流出液中腺苷浓度均为0.05 - 0.3微摩尔/升。流出液中腺苷浓度超过0.05 - 0.03微摩尔/升时，冠脉流量并未增加。这些结果引发了关于腺苷在缺氧灌注期间调节冠脉循环中作用的疑问，尽管在缺氧灌注早期腺苷可能具有血管舒张功能。
