Edvinsson L, Cervós-Navarro J, Larsson L I, Owman C, Rönnberg A L
Neurology. 1977 Sep;27(9):878-83. doi: 10.1212/wnl.27.9.878.
Brain mast cells were studied in mice, rats, rabbits, hamsters, guinea pigs, cats, cows, monkeys, and humans with use of a variety of techniques. They were localized by staining with Astrablau or by toluidine blue-induced metachromasia and characterized by their ultrastructural appearance and by the presence of histochemically demonstrable histamine (o-phthaldiadehyde fluorescence method). The identity of the fluorophore was secured by microspectrofluorometry. Mast cells in brain usually had a perivascular localization but were also found scattered in the parenchyma. The regional variations in the number of mast cells agreed with the histamine concentration as measured fluorometrically. The variation was in the order leptomeninges greater than hypothalamus greater than cerebral cortex = mesencephalon greater than cerebellum = brain stem. In addition to histamine, murine mast cells stored serotonin, whereas bovine mast cells contained dopamine, visualized histochemically by the formaldehyde technique.
运用多种技术对小鼠、大鼠、兔子、仓鼠、豚鼠、猫、牛、猴子和人类的脑肥大细胞进行了研究。通过用阿斯特拉蓝染色或甲苯胺蓝诱导的异染性对其进行定位，并根据其超微结构外观以及组织化学可证实的组胺的存在（邻苯二醛荧光法）对其进行表征。通过显微分光荧光测定法确定荧光团的身份。脑中的肥大细胞通常位于血管周围，但也发现散在于实质中。肥大细胞数量的区域差异与荧光法测量的组胺浓度一致。差异顺序为软脑膜大于下丘脑大于大脑皮层 = 中脑大于小脑 = 脑干。除组胺外，小鼠肥大细胞还储存5-羟色胺，而牛肥大细胞含有多巴胺，通过甲醛技术进行组织化学可视化。
