Huang Shun, Zhao Shuihao, Zhao Haijie, Wen Mingzhang, Guo Zhong
Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education Guangdong Zhuhai-Macao Joint Biotech Laboratory Faculty of Arts and Sciences Beijing Normal University Zhuhai China.
Department of Pharmaceutical Sciences University of Basel Basel Switzerland.
Exploration (Beijing). 2025 Apr 3;5(4):e20240045. doi: 10.1002/EXP.20240045. eCollection 2025 Aug.
There is currently a pressing issue of antimicrobial resistance, with numerous pathogenic superbugs continually emerging, posing significant threats to both human health and the economy. However, the development of new antibiotics has not kept up in pace with the development of microbial resistance, necessitating the exploration of more effective approaches to combat microbes. Synthetic biology offers a novel paradigm by employing selective screening and assembling diverse biological components to redesign biological systems that can specifically target and eliminate microbes. In particular, engineering living therapeutics enables the detection and precise eradication of pathogenic microorganisms in a controlled means. This review provides an overview of recent advancements in engineering living therapeutics using synthetic biology for antibacterial treatment. It focuses on modifying bacteriophages, microbes, and mammalian cells through engineering approaches for antibacterial therapy. The advantages of each approach are delineated along with potential challenges they may encounter. Finally, a prospective outlook is presented highlighting the potential impact and future prospects of this innovative antimicrobial strategy.
目前，抗菌药物耐药性问题紧迫，众多致病性超级细菌不断出现，对人类健康和经济都构成重大威胁。然而，新抗生素的研发未能跟上微生物耐药性的发展步伐，因此有必要探索更有效的抗微生物方法。合成生物学提供了一种新的模式，即通过选择性筛选和组装各种生物组件来重新设计能够特异性靶向和消除微生物的生物系统。特别是，工程化活体治疗剂能够以可控方式检测和精确根除病原微生物。本文综述了利用合成生物学进行抗菌治疗的工程化活体治疗剂的最新进展。它重点介绍了通过工程方法改造噬菌体、微生物和哺乳动物细胞以进行抗菌治疗。阐述了每种方法的优点以及它们可能遇到的潜在挑战。最后，给出了前瞻性展望，突出了这种创新抗菌策略的潜在影响和未来前景。
