Choudhury Swadhin, Pathi Basanti Kumari, Sahoo Jyoti Prakash, Mohanty Shakya, Panigrahi Kumudini
Microbiology, Kalinga Institute of Medical Sciences, Bhubaneswar, IND.
Pharmacology, Kalinga Institute of Medical Sciences, Bhubaneswar, IND.
Cureus. 2025 Jul 27;17(7):e88844. doi: 10.7759/cureus.88844. eCollection 2025 Jul.
Lower respiratory tract infections (LRTIs) increase the morbidity and hospital bed occupancy. Cases of LRTI due to non-fermenting Gram-negative bacilli (NFGNB) are common in healthcare institutions. The common NFGNB found nowadays are , , and . Prophylactic antibiotic use and frequent antimicrobial susceptibility testing (AST) impact the microbes' antimicrobial sensitivity patterns. We planned this study to estimate the prevalence of LRTI due to NFGNB and the antimicrobial sensitivity patterns of the causative microbes.
This retrospective study was conducted at Kalinga Institute of Medical Sciences (KIMS), Bhubaneswar, India. We analyzed data of the patients admitted between June 2023 and May 2025. We included female and male adult patients admitted to KIMS with LRTI during the study period. The specimens of bronchoalveolar lavage (BAL) fluid and endotracheal tube (ET) aspirate obtained from the qualified subjects were examined. Pathogenic bacteria were cultivated using enriched and selective media, such as 5% sheep blood agar and MacConkey agar. To identify isolates and evaluate AST, the VITEK 2 system (bioMérieux, Marcy l'Etoile, France) was employed. To analyze the data, we used R software (version 4.4.3; R Development Core Team, Vienna, Austria).
Our hospital admitted 2,389 patients with LRTI during the research period. Of them, 757 (31.69%) patients had a positive culture result for NFGNB, and 362 (47.82%) participants were female. The study population's median age was 44.79 (38.11-66.43) years. We found three non-fermenters: (401, 52.97%), (248, 32.76%), and (108, 14.27%). The samples of and were highly sensitive to tigecycline. The cases with were mainly sensitive to aztreonam and demonstrated the least sensitivity to colistin.
The NFGNB encountered in this study were , , and . was highly sensitive to aztreonam. and had maximum sensitivity for tigecycline. Future studies with bigger sample sizes must examine the pathogenic NFGNB causing LRTI and their antimicrobial susceptibility patterns.
下呼吸道感染（LRTIs）会增加发病率和医院床位占用率。在医疗机构中，由非发酵革兰氏阴性杆菌（NFGNB）引起的LRTI病例很常见。目前发现的常见NFGNB有 、 和 。预防性使用抗生素和频繁进行抗菌药物敏感性试验（AST）会影响微生物的抗菌敏感性模式。我们开展这项研究以估计由NFGNB引起的LRTI的患病率以及致病微生物的抗菌敏感性模式。
这项回顾性研究在印度布巴内斯瓦尔的卡林加医学科学研究所（KIMS）进行。我们分析了2023年6月至2025年5月期间入院患者的数据。我们纳入了在研究期间因LRTI入住KIMS的成年男女患者。对从符合条件的受试者获取的支气管肺泡灌洗（BAL）液和气管内导管（ET）吸出物标本进行检查。使用富集和选择性培养基（如5%羊血琼脂和麦康凯琼脂）培养病原菌。为了鉴定分离株并评估AST，采用了VITEK 2系统（法国马西伊图瓦勒生物梅里埃公司）。为了分析数据，我们使用了R软件（版本4.4.3；奥地利维也纳R开发核心团队）。
在研究期间，我院收治了2389例LRTI患者。其中，757例（31.69%）患者的NFGNB培养结果呈阳性，362例（47.82%）参与者为女性。研究人群的中位年龄为44.79（38.11 - 66.43）岁。我们发现了三种非发酵菌： （401例，52.97%）、 （248例，32.76%）和 （108例，14.27%）。 和 的样本对替加环素高度敏感。 病例主要对氨曲南敏感，对黏菌素的敏感性最低。
本研究中遇到的NFGNB为 、 和 。 对氨曲南高度敏感。 和 对替加环素的敏感性最高。未来需要更大样本量的研究来检查引起LRTI的致病性NFGNB及其抗菌敏感性模式。
