Ryu Sumin, Doo Hyunok, Kim Eun Sol, Keum Gi Beom, Kwak Jinok, Pandey Sriniwas, Choi Yejin, Kang Juyoun, Kim Sheena, Kim Hyeun Bum, Lee Ju-Hoon
Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea.
Division of Infectious Diseases, Department of Pediatrics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
J Anim Sci Technol. 2025 Jul;67(4):944-947. doi: 10.5187/jast.2023.e134. Epub 2025 Jul 31.
In this study, the complete genome of strain GA_C_14 was sequenced and analyzed. We isolated the strain GA_C_14 from gajami sikhae, the Korean traditional food purchased from a local market in Gangneung, Korea. The genome of the strain GA_C_14 consisted of one circular chromosome (3,196,348bp) with a guanine + cytosine (GC) content of 44.7% and one circular plasmid (40,211bp) with a GC content of 38.9%. The genome annotation revealed 3,083 protein-coding sequences (CDSs), 67 tRNAs, as well as 16 rRNAs. The strain GA_C_14 possesses enzymes involved in vitamin B metabolism, such as (EC 1.2.1.12), (EC 2.6.1.52), (EC 2.2.1.7), (EC 1.1.1.95), (EC 2.7.1.35), and (EC 1.4.3.5). Moreover, the strain GA_C_14 harbors the beta-galactosidase (EC 3.2.1.23) enzyme required for synthesizing galactooligosaccharides (GOS). These results suggest that strain GA_C_14 could be utilized as a functional probiotic in the swine industry.
在本研究中，对GA_C_14菌株的全基因组进行了测序和分析。我们从韩国江陵当地市场购买的韩国传统食品加佳米西克（gajami sikhae）中分离出GA_C_14菌株。GA_C_14菌株的基因组由一条环状染色体（3,196,348bp）组成，鸟嘌呤+胞嘧啶（GC）含量为44.7%，以及一条环状质粒（40,211bp），GC含量为38.9%。基因组注释显示有3,083个蛋白质编码序列（CDS）、67个tRNA以及16个rRNA。GA_C_14菌株拥有参与维生素B代谢的酶，如（EC 1.2.1.12）、（EC 2.6.1.52）、（EC 2.2.1.7）、（EC 1.1.1.95）、（EC 2.7.1.35）和（EC 1.4.3.5）。此外，GA_C_14菌株含有合成低聚半乳糖（GOS）所需的β-半乳糖苷酶（EC 3.2.1.23）。这些结果表明，GA_C_14菌株可作为猪产业中的功能性益生菌加以利用。
