Townsend E C, Xu K, De La Cruz K, Huang L, Sandstrom S, Meudt J J, Shanmuganayagam D, Huttenlocher A, Gibson A L F, Kalan L R
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
mSphere. 2025 Sep 30;10(9):e0044125. doi: 10.1128/msphere.00441-25. Epub 2025 Aug 28.
Incomplete antiseptic efficacy against potentially pathogenic microbial taxa places some patients at disproportionate risk for developing a surgical site infection. Laboratory models capable of interrogating the effects of antiseptics on the skin and its complex microbial communities are desperately needed to improve and better tailor antiseptic formulations. This work aims to establish an porcine skin model to explore the impact of topical antiseptics on complex skin microbial communities and superficial skin lipids. Microbiome samples were treated with propidium monoazide to selectively evaluate DNA from viable microorganisms. Bacterial abundances were assessed via viability-qPCR and quantitative culture. Viable community populations were evaluated with 16S rRNA gene sequencing. Epidermal biopsies were collected at multiple time points for lipidomic assessment via LC/MS. The environment promoted shifts in porcine skin lipid composition and microbial communities over the experiment's duration. Compared to water-treated control skin, skin treated with the antiseptic chlorhexidine gluconate had significantly lower culturable counts and bioburden as determined by viability-qPCR. Compared to water-treated skin, viable microbial communities on CHG-treated skin displayed greater relative abundance of several gut-associated and Gram-negative bacterial taxa, including , , , and . Collectively, these findings highlight the utility of an porcine skin system for interrogating the impacts of antimicrobial disruption on complex microbial ecosystems, and ultimately, for the future testing and development of improved antiseptic formulations.
Broad-spectrum antiseptics are widely used to prevent surgical site infection and as wound cleansing agents. The impacts of such agents on beneficial microbes in the skin microbiome are understudied. Here, we describe an skin model to test the impacts of antiseptics or other topical agents on the healthy skin microbiome.
对潜在致病微生物分类群的抗菌效力不完全，使一些患者面临发生手术部位感染的风险过高。迫切需要能够研究抗菌剂对皮肤及其复杂微生物群落影响的实验室模型，以改进和更好地定制抗菌剂配方。这项工作旨在建立一个猪皮肤模型，以探索局部抗菌剂对复杂皮肤微生物群落和浅表皮肤脂质的影响。微生物组样本用单叠氮化丙锭处理，以选择性评估活微生物的DNA。通过活力定量聚合酶链反应（viability-qPCR）和定量培养评估细菌丰度。用16S rRNA基因测序评估活群落种群。在多个时间点采集表皮活检组织，通过液相色谱/质谱（LC/MS）进行脂质组学评估。在实验过程中，环境促使猪皮肤脂质组成和微生物群落发生变化。与水处理对照皮肤相比，用葡萄糖酸洗必泰处理的皮肤，通过活力定量聚合酶链反应测定，其可培养菌数和生物负荷显著降低。与水处理皮肤相比，洗必泰处理皮肤的活微生物群落显示出几种与肠道相关和革兰氏阴性细菌分类群的相对丰度更高，包括、、和。总的来说，这些发现突出了猪皮肤系统在研究抗菌破坏对复杂微生物生态系统影响方面的实用性，并最终用于改进抗菌剂配方的未来测试和开发。
广谱抗菌剂广泛用于预防手术部位感染和作为伤口清洁剂。此类药物对皮肤微生物群中有益微生物的影响研究不足。在这里，我们描述了一个皮肤模型，以测试抗菌剂或其他局部药物对健康皮肤微生物群的影响。
