Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States.
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States; Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI, United States; Medical Scientist Training Program, University of Wisconsin School of Medicine and Public Health, Madison, WI, United States.
Fungal Genet Biol. 2024 Aug;173:103898. doi: 10.1016/j.fgb.2024.103898. Epub 2024 May 28.
The skin and its microbiome function to protect the host from pathogen colonization and environmental stressors. In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation of bacterial-fungal interactions on this surface. Several diverse bacterial isolates exhibit potent antifungal properties against opportunistic fungal pathogens in vitro. Genomic analysis of inhibitory species revealed a diverse repertoire of uncharacterized biosynthetic gene clusters suggesting a reservoir of novel chemical and biological diversity. Collectively, the porcine skin microbiome represents a potential unique source of novel antifungals.
皮肤及其微生物组的功能是保护宿主免受病原体定植和环境应激源的影响。在这项研究中，我们使用威斯康星州微型猪模型，对猪的皮肤真菌和细菌微生物组进行了特征描述，鉴定了具有抗真菌活性的细菌分离株，并利用全基因组测序鉴定了可能对真菌具有拮抗作用的编码次级代谢产物的生物合成基因簇。通过微生物组测序与培养组学相结合的综合方法，我们报告了发现了新型的棒状杆菌属和罗氏菌属。此外，本研究首次对猪皮肤真菌组进行了全面评估，并评估了该表面的细菌-真菌相互作用。几种不同的细菌分离株在体外对机会性真菌病原体表现出很强的抗真菌特性。抑制性物种的基因组分析揭示了多样化的未表征生物合成基因簇，这表明存在新的化学和生物多样性的潜在储备。总的来说，猪的皮肤微生物组代表了一种潜在的新型抗真菌药物的独特来源。
