Kingsbury Joanne M, French Nigel, Midwinter Anne, Lucas Rebecca, Callander Maree, Hird Cody P, Smith Samantha, Mulqueen Kerry, Biggs Roy, Biggs Patrick J
New Zealand Institute for Public Health and Forensic Science, Christchurch, New Zealand.
New Zealand Food Safety Science and Research Centre, Massey University, Palmerston North, New Zealand.
Appl Environ Microbiol. 2025 Aug 28:e0120625. doi: 10.1128/aem.01206-25.
This longitudinal survey followed the life cycle of a New Zealand broiler flock to investigate sources of flock colonization by . Samples were collected at frequent intervals from potential reservoirs and sources, transmission routes for ingress into the broiler shed, and to monitor flock colonization. Of the 738 samples, 200 (27%) tested positive for species from sample isolates included 316 , 39 and 8 isolates; only was isolated from chickens. isolates ( = 199) were sequenced and consisted of seven sequence types (STs); the most abundant was ST6964 (105 isolates). Most flock isolates were ST6964 (44 isolates) or ST50 (27 isolates). ST6964 isolates closely matched those from the previous flock and another age-matched flock on the same farm, supporting a role for an on-farm reservoir contaminating flocks. There were six STs from catching crew and equipment isolates; the most prevalent were ST6964 (19 isolates) and ST50 (21 isolates). The close genetic match, high prevalence in catching samples (59/130, 45%), and the timing of flock colonization occurring closely following catcher presence in the shed support that catchers and equipment might also contaminate the shed and flock from prior flocks that they visited. There was no evidence for wildlife, feed, drinking water, breeder flock, or shed litter as sources of the genotypes colonizing the flock. Taken together, this study identified key areas where the poultry industry might focus on-farm risk management practices to reduce colonization of broiler flocks by .IMPORTANCECampylobacteriosis is the most frequently notified enteric disease in New Zealand, and New Zealand has one of the highest rates of campylobacteriosis among industrialized countries. Reducing colonization of poultry at the farm level would reduce reliance on processing interventions for reducing contamination of broiler meat. This study aimed to identify on-farm sources of contamination in New Zealand broiler chicken flocks. No evidence was found that wildlife, chicken feed, drinking water, or parent breeder flocks were contaminating sources. Instead, carryover of from the previous flock or other farm flocks, and/or contamination from chicken catching crews and their equipment, may have contributed strains that colonized the study flock. These are key areas where the poultry industry might focus on-farm risk management practices to reduce colonization of broiler flocks by .
这项纵向调查跟踪了新西兰一个肉鸡群的生命周期，以调查该群鸡定植的来源。从潜在储存库和源头、进入肉鸡舍的传播途径等方面频繁采集样本，并监测鸡群定植情况。在738份样本中，200份（27%）检测出某种菌呈阳性，样本分离株中的菌种包括316株、39株和8株；仅从鸡中分离出该菌。对该菌的199株分离株进行了测序，共包含7种序列类型（STs）；最常见的是ST6964（105株）。大多数鸡群分离株为ST6964（44株）或ST50（27株）。ST6964分离株与前一批鸡群以及同一农场另一个年龄匹配鸡群的分离株密切匹配，这支持了农场储存库污染鸡群的作用。捕捉人员和设备分离株有6种STs；最常见的是ST6964（19株）和ST50（21株）。基因匹配度高、捕捉样本中该菌的高流行率（59/130，45%）以及鸡群定植时间紧随捕捉人员进入鸡舍之后，这些都表明捕捉人员和设备可能也会将他们之前去过的鸡群中的菌株污染鸡舍和鸡群。没有证据表明野生动物、饲料、饮用水、种鸡群或鸡舍垫料是定植该鸡群的该菌基因型的来源。综上所述，本研究确定了家禽业在农场风险管理实践中可能重点关注的关键领域，以减少肉鸡群被该菌定植。
弯曲杆菌病是新西兰报告最多的肠道疾病，在工业化国家中，新西兰的弯曲杆菌病发病率位居前列。在农场层面减少家禽被弯曲杆菌定植，将减少对加工干预措施的依赖，以降低肉鸡胴体的弯曲杆菌污染。本研究旨在确定新西兰肉鸡群中弯曲杆菌污染的农场来源。未发现野生动物、鸡饲料、饮用水或种鸡群是污染源的证据。相反，前一批鸡群或其他农场鸡群中的弯曲杆菌残留，和/或抓鸡人员及其设备的污染，可能导致了定植本研究鸡群的菌株。这些是家禽业在农场风险管理实践中可能重点关注的关键领域，以减少肉鸡群被弯曲杆菌定植。
