Maguire Mark, Serna Carlos, Delgado-Blas Jose F, Clarke Christina, DeLappe Niall, Cormican Martin, Coughlan Simone C, Miliotis Georgios, Gonzalez-Zorn Bruno, Burke Liam P
Antimicrobial Resistance and Microbial Ecology Group, School of Medicine, University of Galway, Galway, Ireland.
Centre for One Health, Ryan Institute, University of Galway, Galway, Ireland.
Microb Genom. 2025 Aug;11(8). doi: 10.1099/mgen.0.001473.
Aminoglycosides are used in the treatment of serious infections with Gram-negative bacteria, especially those resistant to beta-lactams and carbapenems. 16S rRNA methyltransferases (16S-RMTase) are capable of conferring resistance to nearly all aminoglycosides. They are sometimes detected in combination with . This study describes the mobile genetic elements associated with and 16S-RMTase (co-)carriage in from Ireland in the period 2019-2023. All isolates (=58) carrying both and a 16S-RMTase gene between 2019 and 2023 were obtained from the CPE National Reference Laboratory Service. Short-read sequences were generated for all isolates, and long-read sequences were generated for a subset of isolates (=27). MOB-recon was used to distinguish plasmid-derived contigs from draft assemblies. The containment distance and DCJ-indel distance were used to find clusters of related plasmids. Isolates carrying were associated with (=31) but also (=6) carriage. These genes were co-localized most frequently on IncFIB/HI1B (=12), IncM2 (=10) and IncC (=8) plasmids. Closely related plasmids were identified in multiple species (range: 2-5) and at different sites around Ireland; however, the IncM2 plasmids were largely associated with a single hospital. Isolates carrying were associated with (=28) carriage. The majority (=15) were carried on a diverse range of mosaic IncF-type plasmids. Two discrete clusters of IncM1 (=3) and IncFII (=4) type plasmids were also detected. The study highlights the diverse plasmids co-carrying carbapenem and aminoglycoside resistance genes in Ireland. Detection of plasmids across multiple species and hospitals suggests dissemination driven by antimicrobial selective pressure and environmental reservoirs within healthcare networks. The co-dissemination of these genes on highly mobile plasmids poses a significant public health concern and emphasizes the need for greater awareness that chains of transmission of antimicrobial resistance in the healthcare setting may involve multiple species.
氨基糖苷类药物用于治疗革兰氏阴性菌引起的严重感染，尤其是对β-内酰胺类和碳青霉烯类耐药的感染。16S rRNA甲基转移酶（16S-RMTase）能够赋予对几乎所有氨基糖苷类药物的耐药性。它们有时与……一起被检测到。本研究描述了2019年至2023年期间来自爱尔兰的……与16S-RMTase（共）携带相关的移动遗传元件。2019年至2023年期间所有同时携带……和16S-RMTase基因的分离株（=58株）均来自碳青霉烯类耐药肠杆菌科细菌国家参考实验室服务机构。对所有分离株生成短读长序列，对一部分分离株（=27株）生成长读长序列。使用MOB-recon从草图组装中区分质粒衍生的重叠群。使用包含距离和DCJ插入缺失距离来寻找相关质粒的簇。携带……的分离株与……（=31株）相关，但也与……（=6株）携带相关。这些基因最常共定位在IncFIB/HI1B（=12）、IncM2（=10）和IncC（=8）质粒上。在多个物种（范围：2 - 5个）以及爱尔兰各地不同地点鉴定出密切相关的质粒；然而，IncM2质粒主要与一家医院相关。携带……的分离株与……（=28株）携带相关。大多数（=15株）携带在多种嵌合IncF型质粒上。还检测到两个离散的IncM1（=3株）和IncFII（=4株）型质粒簇。该研究突出了爱尔兰共携带碳青霉烯类和氨基糖苷类耐药基因的多种质粒。在多个物种和医院中检测到质粒表明，抗菌选择压力和医疗保健网络内的环境储存库推动了质粒的传播。这些基因在高度移动的质粒上的共同传播构成了重大的公共卫生问题，并强调需要提高认识，即医疗保健环境中抗菌药物耐药性的传播链可能涉及多个物种
