Shahriari Mahshad, Nejad Ebrahimi Samad, Rahimi Masoud, Khademian Azadeh, Ardekani Mahsa Motamedi, Alves Rita C, Oliveira Maria Beatriz P P, Fathi Faezeh
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran.
Int J Biol Macromol. 2025 Sep;323(Pt 2):147164. doi: 10.1016/j.ijbiomac.2025.147164. Epub 2025 Aug 26.
Pomegranate juice (PJ), rich in anthocyanins (ACNs), was efficiently concentrated using a nonionic acrylic polymeric adsorbent resin to develop ACNs-rich fraction aim to develop natural pH-sensitive indicators for real-time food deterioration detection. The ACNs-rich fraction was incorporated into fiber films made from two hydrocolloid matrices composed of polyvinyl alcohol, chitosan, and starch. Optimal adsorption conditions (94.0 %) were achieved at pH 2.5, with a 40-minute contact time and a PJ-to-resin ratio of 30 ml/0.7 g. Desorption reached 93.3 % using a 6-bed volume solvent and 8-minute contact time. HPLC analysis showed an 82-fold increase in anthocyanin content and a 6.2-fold rise in antioxidant activity compared to raw PJ. Isotherm and kinetic studies showed good fits with the Freundlich (R = 0.96) and pseudo-second-order (R = 0.99) models, respectively. FTIR-ATR and molecular docking analyses revealed hydrogen bonding between ACNs and the polymer matrix. The developed fiber films based on chitosan and polyvinyl alcohol exhibited ultrafine average diameters, pronounced color changes in response to ammonia exposure, and antioxidant activities that were 51.0 and 72.5 times higher than those of the unloaded control. This study introduces a novel natural indicator for real-time food deterioration and proposes optimized, scalable preparation methods with commercial potential for food packaging.
石榴汁（PJ）富含花青素（ACNs），使用非离子型丙烯酸类聚合物吸附树脂对其进行高效浓缩，以获得富含ACNs的馏分，旨在开发用于实时食品变质检测的天然pH敏感指示剂。将富含ACNs的馏分掺入由聚乙烯醇、壳聚糖和淀粉组成的两种水胶体基质制成的纤维膜中。在pH 2.5、接触时间40分钟、PJ与树脂比例为30 ml/0.7 g的条件下，实现了94.0%的最佳吸附率。使用6倍床体积的溶剂和8分钟的接触时间，解吸率达到93.3%。高效液相色谱分析表明，与未加工的PJ相比，花青素含量增加了82倍，抗氧化活性提高了6.2倍。等温线和动力学研究分别与Freundlich模型（R = 0.96）和伪二级模型（R = 0.99）拟合良好。傅里叶变换红外光谱-衰减全反射（FTIR-ATR）和分子对接分析揭示了ACNs与聚合物基质之间的氢键。基于壳聚糖和聚乙烯醇开发的纤维膜具有超细的平均直径，暴露于氨时颜色变化明显，抗氧化活性分别比未负载对照高51.0倍和72.5倍。本研究引入了一种用于实时食品变质的新型天然指示剂，并提出了具有食品包装商业潜力的优化、可扩展制备方法。
