Raettig R, Kersten H, Weissenbach J, Dirheimer G
Nucleic Acids Res. 1977 Jun;4(6):1769-82. doi: 10.1093/nar/4.6.1769.
tRNA (adenine-1) methyltransferase occurs in Bacillus subtilis. Eucaryotic tRNAThr and tRNATyr from yeast in which 1-methyladenosine (m1A) is already present in the TpsiC loop, can be methylated in vitro with S-adenosylmethionine and B. subtilis extracts. Each of the specific tRNAs accepts 1 mol of methyl groups per mol tRNA. The enzyme transforms into m1A the 3'-terminal adenylic acid residue of the dihydrouridine loop, a new position for a modified adenosine residue in tRNA. Both tRNAs have the sequence Py-A-A-G-G-C-m2(2)G in the D-loop and D-stem region. Other tRNAs with the same sequence in this region also serve as substrates for the tRNA (adenine-1) methyltransferase.
tRNA（腺嘌呤-1）甲基转移酶存在于枯草芽孢杆菌中。来自酵母的真核生物苏氨酸tRNA和酪氨酸tRNA，其TψC环中已存在1-甲基腺苷（m1A），可以在体外与S-腺苷甲硫氨酸和枯草芽孢杆菌提取物一起进行甲基化。每种特定的tRNA每摩尔tRNA接受1摩尔甲基。该酶将二氢尿嘧啶环的3'-末端腺苷酸残基转化为m1A，这是tRNA中修饰腺苷残基的一个新位置。这两种tRNA在D环和D茎区域具有Py-A-A-G-G-C-m2(2)G序列。在该区域具有相同序列的其他tRNA也可作为tRNA（腺嘌呤-1）甲基转移酶的底物。
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