枯草芽孢杆菌腺嘌呤-1-甲基转移酶对二氢尿嘧啶环特异识别原核生物和真核生物的转移核糖核酸。
Recognition of individual procaryotic and eucaryotic transfer-ribonucleic acids by B subtilis adenine-1-methyltransferase specific for the dihydrouridine loop.
作者信息
Kersten H, Raettig R, Weissenbach J, Dirheimer G
出版信息
Nucleic Acids Res. 1978 Aug;5(8):3033-42. doi: 10.1093/nar/5.8.3033.
Abstract
Bulk tRNA from yeast and Rat liver can be methylated in vitro with -adenosylmethionine and B, subtilis extracts. The sole product formed is 1-methyladenosine (m1A). This tRNA (adenine-1) methyltransferase converts quantitatively the 3'-terminal adenosine-residue in the dihydrouridine-loop of tRNAThr and tRNATyr from yeast into m1A. Out of 16 eucaryotic tRNAs with known sequences 6 accepted methyl groups, all at a molar ratio of 1. These tRNAs have in common an unpaired adenosine-residue at the specific site in the sequence Py-A-A+-G-G-C-m2G. Out of 12 tRNAs from E. coli 6 served as specific substrates. These E. coli tRNAs also have an unpaired adenosine-residue at the 3'-end of the D-loop. Besides restrictions in primary structure intact secondary and tertiary structure is important for recognition of the specific tRNAs by the enzyme.
摘要
来自酵母和大鼠肝脏的大量tRNA可在体外与S-腺苷甲硫氨酸和枯草芽孢杆菌提取物一起进行甲基化。形成的唯一产物是1-甲基腺苷（m1A）。这种tRNA（腺嘌呤-1）甲基转移酶将酵母tRNAThr和tRNATyr二氢尿嘧啶环中的3'-末端腺苷残基定量转化为m1A。在16种已知序列的真核tRNA中，有6种接受甲基基团，所有甲基的摩尔比均为1。这些tRNA在序列Py-A-A+-G-G-C-m2G的特定位点有一个未配对的腺苷残基。在12种来自大肠杆菌的tRNA中，有6种作为特异性底物。这些大肠杆菌tRNA在D环的3'-末端也有一个未配对的腺苷残基。除了一级结构的限制外，完整的二级和三级结构对于该酶识别特定的tRNA也很重要。
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2
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3
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Proc Natl Acad Sci U S A. 1966 Apr;55(4):941-8. doi: 10.1073/pnas.55.4.941.
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