Pabuayon Isaiah Catalino M, Rashid Md Mamunur, Kitazumi Ai, Cushman Kevin R, Ressom Habtom W, De Los Reyes Benildo G
Department of Plant and Soil Science, Davis College of Agricultural Sciences and Natural Resources, Texas Tech University, Lubbock, TX, USA.
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.
Planta. 2025 Aug 29;262(4):92. doi: 10.1007/s00425-025-04811-0.
Understanding the nature of non-parental phenotypes created by transgressive segregation is important in creating novel genetic recombinants that can withstand different environmental conditions for crop production. FL510, a transgressive salinity-tolerant rice genotype from a cross between IR29 (salt-sensitive) and Pokkali (salt-tolerant), has tolerance mechanisms active under control conditions and improves survival upon the onset of salinity. This study compares normal-state metabolomes and lipidomes of FL510 with its parents. Principal component analysis (PCA) of the identified analytes showed clear and expected similarity between FL510 and Pokkali, while partial least squares discriminant analysis (PLS-DA) emphasized overlaps between the metabolic profiles of IR29 and FL510. The analysis identified metabolites with inherited patterns of abundance from either parent in FL510 and those with unique, non-parental abundances, and these were supported by differential expression of key pathway-related genes identified through transcriptome analysis. Strigolactone precursor production was identified as a key feature in FL510, which may help explain its unique architecture that is beneficial for osmotic stress. We also identified a divergence between productivity under ideal environments leading to free radical production versus tempered production that offers better survival under marginal growing conditions. FL510 showed an inheritance of hormone and amino acid abundances from Pokkali, which further explains some of its architectural and previously studied stress-response features. Meanwhile, the similarity of FL510 with IR29 in terms of flavonoid indicates an inheritance of productivity and is consistent with previous reports of induction for these molecules under stress, rather than being active under control conditions. MAIN CONCLUSION: Through repeated genetic recombination of genetically distant alleles, the transgressive segregant FL510 gained unique, non-parental signaling pathways and complementary metabolome features from both parents leading to positive net genetic gains.
了解通过超亲分离产生的非亲本表型的本质，对于创造能够在不同环境条件下用于作物生产的新型基因重组体至关重要。FL510是一种耐盐性超亲水稻基因型，由IR29（盐敏感型）和Pokkali（耐盐型）杂交产生，其耐受机制在对照条件下就已激活，并在盐胁迫开始时提高存活率。本研究比较了FL510与其亲本的正常状态代谢组和脂质组。对已鉴定分析物的主成分分析（PCA）表明，FL510和Pokkali之间存在明显且预期的相似性，而偏最小二乘判别分析（PLS-DA）则强调了IR29和FL510代谢谱之间的重叠。该分析确定了FL510中从任一亲本继承的丰度模式的代谢物，以及具有独特的、非亲本丰度的代谢物，并且通过转录组分析鉴定的关键途径相关基因的差异表达也支持了这些结果。独脚金内酯前体的产生被确定为FL510的一个关键特征，这可能有助于解释其有利于渗透胁迫的独特结构。我们还发现，在理想环境下导致自由基产生的生产力与在边际生长条件下提供更好存活率的适度生产力之间存在差异。FL510表现出从Pokkali继承的激素和氨基酸丰度，这进一步解释了其一些结构特征以及先前研究的应激反应特征。同时，FL510在类黄酮方面与IR29的相似性表明了生产力的继承，并且与先前关于这些分子在胁迫下被诱导而非在对照条件下活跃的报道一致。主要结论：通过对遗传距离较远的等位基因进行反复的基因重组，超亲分离系FL510从双亲获得了独特的、非亲本的信号通路和互补的代谢组特征，从而实现了正向的净遗传增益。
