Zhang Danfeng, Tian Xinrui, Hu Tingting, Wang Yanqiao, Wang Yonghai, Hong Changqing, Shi Wei, Ye Yingwang
Engineering Research Center of Bio-Process, Ministry of Education, School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230601, Anhui, China.
School of Civil and Hydraulic Engineering, Hefei University of Technology, Hefei, 230009, China.
Antonie Van Leeuwenhoek. 2025 Aug 29;118(10):144. doi: 10.1007/s10482-025-02154-0.
Aspergillus flavus infenction of crops, which are common worldwide, are a significant threat to human and animal health. In this study, the antagonistic effects of Pantoea vagans strain BWL1 on A. flavus were analyzed. BWL1 effectively inhibited A. flavusconidial germination, vegetative growth, and sporulation. Notably, A. flavus infections and aflatoxin contents decreased in peanut and maize samples treated with BWL1. A GC-MS analysis and a two-sealed-base-plates assay showed that BWL1 can produce several antifungal volatiles, including 2,5-dimethyl-pyrazine, 2-ethyl-1-hexanol, hexamethyl-cyclotrisiloxane, phenylethyl alcohol, 2,4-di-tert-butylphenol, pentadecane, tetradecane, heptadecane, and n-hexadecanoic acid, with MICs of 0.2, 0.8, 16, 0.8, 0.2, 8, 8, 8, and 8 g/L, respectively. Among these volatiles, 2,5-dimethyl-pyrazine, 2-ethyl-1-hexanol, phenylethyl alcohol, and 2,4-di-tert-butylphenol had substantial inhibitory effects on A. flavus infection of peanuts and maize kernels. The study findings suggest that P. vagans BWL1 may be an potential biocontrol agent for the postharvest management of A. flavus in crops. This is the first report regarding the antifungal effects of P. vagans BWL1 on A. flavus.
黄曲霉对农作物的感染在全球范围内普遍存在，对人类和动物健康构成重大威胁。在本研究中，分析了泛菌属菌株BWL1对黄曲霉的拮抗作用。BWL1有效抑制了黄曲霉的分生孢子萌发、营养生长和孢子形成。值得注意的是，用BWL1处理的花生和玉米样品中的黄曲霉感染和黄曲霉毒素含量降低。气相色谱-质谱分析和双密封底板试验表明，BWL1可产生多种抗真菌挥发物，包括2,5-二甲基吡嗪、2-乙基-1-己醇、六甲基环三硅氧烷、苯乙醇、2,4-二叔丁基苯酚、十五烷、十四烷、十七烷和正十六烷酸，其最低抑菌浓度分别为0.2、0.8、16、0.8、0.2、8、8、8和8 g/L。在这些挥发物中，2,5-二甲基吡嗪、2-乙基-1-己醇、苯乙醇和2,4-二叔丁基苯酚对花生和玉米粒的黄曲霉感染具有显著的抑制作用。研究结果表明，泛菌BWL1可能是一种用于农作物采后黄曲霉管理的潜在生物防治剂。这是关于泛菌BWL1对黄曲霉抗真菌作用的首次报道。
