Qian Xifeng, Deng Yuanrui, Guo Tingting, Huang Xin, Yan Chaowu, Gao Xin, Wu Yan, Yan Xinxin, Liu Zhiqiang, Hu Song, Tan Jiangshan, Chong Lingtao, Zhu Shengsong, Ma Mingjie, Ye Mengting, Hua Lu, Cao Jian, Wang Xiaojian
Key Laboratory of Pulmonary Vascular Medicine, Department of Cardiology, National Clinical Research Center of Cardiovascular Diseases, National Center for Cardiovascular Diseases, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100037, China.
Department of Cardiology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, 100853, China.
Metabolomics. 2025 Aug 29;21(5):130. doi: 10.1007/s11306-025-02317-0.
Right heart (RH), as a junction between the venous system and pulmonary circulation, gains great emphasis on exploring the relevant pathological mechanism of many cardiopulmonary diseases. Although these pathogensis researches centering on RH-related diseases advance, the physiological mechanism research of the RH is scarce.
This study aimed to accurately unravel the metabolic features of normal trans-RH through non-targeted metabolomics.
Patent foramen ovale (PFO) participants with normal function of RH were recruited and their blood samples from superior vena cava (SVC) and pulmonary artery (PA) were collected through right cardiac catheterization. Non-targeted metabolomics analysis based on UHPLC-MS/MS was utilized to generate the metabolic feature of trans-RH by comparing the metabolites change from SVC to PA, revealing its physiological gradient metabolic mechanism.
1060 metabolites were tentatively identified in blood samples from 28 PFO participants. 51 differential metabolites were defined based on screening criteria after flowing through RH, including 39 down-regulated metabolites and 12 up-regulated metabolites. Among them, phosphatidylcholines, sphingomyelins, amino acids, triacylglycerol, neopterin, and tetradecanedioic acid were the most relevant.
Our study provides a more profound and extensive understanding of the psychological metabolism of trans-RH, expanding the current knowledge of normal RH function and providing clues for the pathogenesis research of RH-related diseases.
右心作为静脉系统与肺循环的连接部位，在探索许多心肺疾病的相关病理机制方面受到高度重视。尽管围绕右心相关疾病的发病机制研究不断推进，但右心的生理机制研究却很匮乏。
本研究旨在通过非靶向代谢组学准确揭示正常经右心的代谢特征。
招募右心功能正常的卵圆孔未闭（PFO）参与者，通过右心导管插入术采集他们上腔静脉（SVC）和肺动脉（PA）的血样。利用基于超高效液相色谱-串联质谱（UHPLC-MS/MS）的非靶向代谢组学分析，通过比较从SVC到PA的代谢物变化来生成经右心的代谢特征，揭示其生理梯度代谢机制。
在28名PFO参与者的血样中初步鉴定出1060种代谢物。根据流经右心后的筛选标准确定了51种差异代谢物，包括39种下调代谢物和12种上调代谢物。其中，磷脂酰胆碱、鞘磷脂、氨基酸、三酰甘油、新蝶呤和十四烷二酸最为相关。
我们的研究为经右心的生理代谢提供了更深入广泛的理解，扩展了当前对正常右心功能的认识，并为右心相关疾病的发病机制研究提供了线索。
