Whitfield Hayley L, Sprigg Colleen, Riley Andrew M, Shipton Megan L, Potter Barry V L, Brearley Charles A
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, UK.
Medicinal Chemistry & Drug Discovery, Department of Pharmacology, University of Oxford, Oxford, UK.
Methods Mol Biol. 2025;2972:61-79. doi: 10.1007/978-1-0716-4799-8_6.
High-performance anion-exchange chromatography with acid eluents allows for effective separation of myo-inositol tetrakisphosphates, myo-inositol pentakisphosphates, diphospho-myo-inositol phosphates (inositol pyrophosphates), inositol hexakisphosphates, inositol pyrophosphate analogs, and carbohydrate-based analogs of inositol phosphates. All are detectable by postcolumn addition of ferric ion. In the following, we provide examples drawn from characterization of ITPK, IPK1, and IP 3K enzymes.
使用酸性洗脱液的高效阴离子交换色谱法能够有效分离肌醇四磷酸、肌醇五磷酸、二磷酸肌醇磷酸（肌醇焦磷酸）、肌醇六磷酸、肌醇焦磷酸类似物以及肌醇磷酸的碳水化合物基类似物。通过柱后添加铁离子均可检测到这些物质。以下，我们提供了从ITPK、IPK1和IP 3K酶的表征中获取的示例。
