Rana Priyanshi, Ghosh Riya, Laha Debabrata
Department of Biochemistry, Indian Institute of Science (IISc), Bengaluru, India.
Methods Mol Biol. 2025;2972:51-60. doi: 10.1007/978-1-0716-4799-8_5.
Inositol pyrophosphates are diphosphate-containing inositol phosphate cellular messengers that orchestrate a large array of physiological processes in eukaryotes. In plants, these energy-rich second messengers are involved in hormone signaling and phosphate homeostasis. Notably, much of our understanding of the functional roles of inositol pyrophosphates in plants is based on research involving the model organism Arabidopsis thaliana. Emerging recent studies have highlighted the possible functional importance of these PP-InsP species across plant kingdom. Specifically, PP-InsPs are linked to nutrient sensing and thallus development in Marchantia polymorpha, a nonvascular bryophyte that first emerged on land around 500 million years ago. In this study, we employed strong anion-exchange (SAX)-HPLC techniques combined with [H]-myo-inositol labeling to analyze the steady-state cellular levels of various InsP and PP-InsP species of M. polymorpha.
肌醇焦磷酸是含有二磷酸的肌醇磷酸细胞信使，可协调真核生物中的一系列生理过程。在植物中，这些富含能量的第二信使参与激素信号传导和磷酸盐稳态。值得注意的是，我们对肌醇焦磷酸在植物中功能作用的许多理解都基于对模式生物拟南芥的研究。最近出现的研究强调了这些焦磷酸肌醇物种在整个植物界可能具有的功能重要性。具体而言，焦磷酸肌醇与地钱（一种约5亿年前首次出现在陆地上的非维管苔藓植物）的营养感知和叶状体发育有关。在本研究中，我们采用强阴离子交换（SAX）-高效液相色谱技术结合[H]-肌醇标记来分析地钱各种肌醇磷酸和焦磷酸肌醇物种的稳态细胞水平。
