[矿质盐对苏云金芽孢杆菌培养物外毒素形成及生产力的影响]

[Effect of mineral salts on exotoxin formation and productivity of a Bacillus thuringiensis culture].

作者信息

Abrosimova L I, Babaeva P V, Zubareva G M, Shevtsov V V

出版信息

Mikrobiologiia. 1986 May-Jun;55(3):440-4.

Abstract

The effect of ten mineral salts on the productivity and toxin synthesis was studied in Bacillus thuringiensis IPM-1140. Exotoxin synthesis was stimulated by Zn2+, Mn2+ and NH4+ ions as well as by potassium phosphates. The direct correlation between the number of viable spores and the exotoxin accumulation was disordered at extreme salt concentrations. Optimal salt concentrations in the yeast-polysaccharide medium were found using the method of a fractional factor experiment, which made it possible to increase the productivity of the culture to 5 X 10(9) spores/ml and the yield of the exotoxin to 730 micrograms/ml. The thermoresistance and the entomopathogenic activity of crystals increased when B. thuringiensis IPM-1140 was grown in this medium.

摘要

研究了十种矿物盐对苏云金芽孢杆菌IPM - 1140生产力和毒素合成的影响。锌离子、锰离子、铵离子以及磷酸钾能刺激外毒素的合成。在极端盐浓度下，活芽孢数量与外毒素积累之间的直接相关性被打乱。采用分数因子实验法找到了酵母 - 多糖培养基中的最佳盐浓度，这使得培养物的生产力提高到5×10⁹个芽孢/毫升，外毒素产量提高到730微克/毫升。当苏云金芽孢杆菌IPM - 1140在此培养基中生长时，晶体的耐热性和昆虫致病活性增强。

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