Wang Luyao, Wen Shengxuan, Jiang Qi, Yan Minglong, Li Ying, Gang Biyao, Wang Yucheng, Guo Huiyan
College of Forestry, Shenyang Agricultural University, Shenyang, Liaoning 110866, China; The Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
College of Forestry, Shenyang Agricultural University, Shenyang, Liaoning 110866, China; The Key Laboratory of Forest Tree Genetics, Breeding and Cultivation of Liaoning Province, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
Int J Biol Macromol. 2025 Sep;323(Pt 1):147184. doi: 10.1016/j.ijbiomac.2025.147184. Epub 2025 Aug 27.
Soil salinity represents a major abiotic stress factor that inhibits plant growth and causes substantial yield losses. NAC transcription factors (TFs) play essential roles in plant biological processes and stress responses; however, their functions and underlying mechanisms in salt stress response remain inadequately understood. In this study, we characterized a PdbNAC1 TF from P. davidiana × P. bolleana. Through overexpression and knockout studies of PdbNAC1, we established that its expression positively correlates with salt tolerance through enhanced ROS scavenging, regulation of osmotic and ionic levels, stomatal aperture control, and lignin and cellulose biosynthesis. Furthermore, PdbNAC1 regulates the expression of target genes associated with ROS accumulation, proline synthesis, photosynthesis, lignin and cellulose biosynthesis, ion balance, and stomatal closure regulation, as confirmed by qRT-PCR and RNA-seq analyses. Additionally, PdbNAC1 interacts with two novel and two known functional elements to activate target gene expression, as validated through TF-Centered Y1H, ChIP, Y1H and GUS activity assays. These results provide significant insights into the roles and regulatory mechanisms of NAC TFs. This research highlights PdbNAC1's crucial regulatory role in salt tolerance and presents a potential approach for the genetic enhancement of salt-resistant plants.
土壤盐渍化是一种主要的非生物胁迫因素，它会抑制植物生长并导致大量产量损失。NAC转录因子（TFs）在植物生物学过程和胁迫反应中发挥着重要作用；然而，它们在盐胁迫反应中的功能和潜在机制仍未得到充分了解。在本研究中，我们对来自山桃×碧桃的PdbNAC1转录因子进行了表征。通过对PdbNAC1的过表达和敲除研究，我们确定其表达通过增强活性氧清除、调节渗透和离子水平、控制气孔孔径以及木质素和纤维素生物合成与耐盐性呈正相关。此外，qRT-PCR和RNA-seq分析证实，PdbNAC1调节与活性氧积累、脯氨酸合成、光合作用、木质素和纤维素生物合成、离子平衡以及气孔关闭调节相关的靶基因的表达。此外，通过以转录因子为中心的酵母单杂交（Y1H）、染色质免疫沉淀（ChIP）、Y1H和GUS活性测定验证，PdbNAC1与两个新的和两个已知的功能元件相互作用以激活靶基因表达。这些结果为NAC转录因子的作用和调控机制提供了重要见解。本研究突出了PdbNAC1在耐盐性中的关键调控作用，并为抗盐植物的遗传改良提供了一种潜在方法。
