用于容积性肌肉损失临时治疗的负载维生素E的肌肉填充剂的体内评估。
In-vivo evaluation of vitamin E loaded muscle void fillers for the provisional treatment of volumetric muscle loss.
作者信息
Valerio Michael S, Clark Andrew R, Behun Marissa N, Dearth Christopher L, Goldman Stephen M
机构信息
Extremity Trauma and Amputation Center of Excellence, Defense Health Agency, Falls Church, VA, USA.
Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
出版信息
BMC Res Notes. 2025 Aug 29;18(1):372. doi: 10.1186/s13104-025-07454-2.
OBJECTIVE
This study investigates the therapeutic potential of α-tocopherol (Vitamin E, VitE), delivered locally via a polyethylene glycol (PEG) hydrogel serving as a muscle void filler (MVF), for the acute treatment of volumetric muscle loss (VML) in a rat model. The primary goal was to determine if VitE, a reactive oxygen species scavenger, could exert antioxidant effects at the VML site and thereby improve the recovery of adjacent muscle tissue over a four-week period.
RESULTS
The MVF successfully increased VitE levels in the muscle throughout the implantation period. However, only the lowest concentration of VitE (5 mg/ml) showed a demonstrable effect, resulting in a 30% reduction in peak force compared to the PEG-alone group and a median muscle fiber size that was 4.34 μm smaller. Biochemical analysis revealed no differences in the expression of TGFβ1, protein carbonyl, or malondialdehyde across any groups. While local delivery of VitE was successful, further research is needed to optimize dosing and investigate additional mechanisms for improved muscle recovery.
目的
本研究探讨了通过作为肌肉缺损填充剂（MVF）的聚乙二醇（PEG）水凝胶局部递送α-生育酚（维生素E，VitE）对大鼠模型中容积性肌肉损失（VML）进行急性治疗的潜力。主要目标是确定作为活性氧清除剂的VitE是否能在VML部位发挥抗氧化作用，从而在四周内改善相邻肌肉组织的恢复情况。
结果
在整个植入期，MVF成功提高了肌肉中的VitE水平。然而，只有最低浓度的VitE（5毫克/毫升）显示出明显效果，与单独使用PEG的组相比，峰值力降低了30%，且肌纤维大小中位数小4.34微米。生化分析显示，各实验组之间转化生长因子β1（TGFβ1）、蛋白质羰基或丙二醛的表达无差异。虽然VitE的局部递送是成功的，但仍需要进一步研究来优化给药剂量，并研究改善肌肉恢复的其他机制。
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