Taranova N P
Neurochem Res. 1985 Nov;10(11):1483-97.
EAE in rabbits was induced by means of inoculation of purified myelin of homologous spinal cord with complete Freund's adjuvant. The content of all the major lipid classes was studied by biochemical and histochemical methods in the different parts of spinal cord and in the brain stem in combination with morphological control for the demyelinating process presence. The most expressed myelin damage was found in the lumbar and sacral parts of spinal cord. In the same parts the content of phospholipids, cerebrosides, and free cholesterol decreased and cholesterol esters were shown to accumulate. Histochemical analysis supported these findings and revealed that the loss of lipids occurred directly in the demyelination foci. Changes in total ganglioside content and in ganglioside fractions ratio were not observed. In the brain stem neither morphological, nor biochemical changes were found. On the basis of these data it was concluded that pathological processes of periaxonal demyelination, induced by the sensitization with purified myelin, have not damaged neuronal structures and not involved the brain.
通过用完全弗氏佐剂接种同源脊髓的纯化髓磷脂在兔中诱导实验性自身免疫性脑脊髓炎（EAE）。采用生化和组织化学方法研究了脊髓不同部位和脑干中所有主要脂质类别的含量，并结合脱髓鞘过程存在的形态学对照。在脊髓的腰段和骶段发现了最明显的髓磷脂损伤。在相同部位，磷脂、脑苷脂和游离胆固醇的含量降低，胆固醇酯积累。组织化学分析支持了这些发现，并表明脂质的损失直接发生在脱髓鞘病灶中。未观察到总神经节苷脂含量和神经节苷脂组分比例的变化。在脑干中未发现形态学和生化变化。基于这些数据得出结论，用纯化髓磷脂致敏诱导的轴周脱髓鞘病理过程未损害神经元结构，也未累及脑。
