Scott-Baumann Jim, Watson Alice H, Mur Luis, Syme Harriet M
Aberystwyth University, Faculty of Earth and Life Sciences, Aberystwyth School of Veterinary Sciences, Aberystwyth, Wales, United Kingdom of Great Britain and Northern Ireland.
Royal Veterinary College, Deptartment Clinical Science and Services, Hertfordshire, United Kingdom of Great Britain and Northern Ireland.
J Vet Intern Med. 2025 Sep-Oct;39(5):e70227. doi: 10.1111/jvim.70227.
Early diagnosis of hypertension remains an important problem in cats. Lack of routine blood pressure screening in primary care practice, and the possibility of white coat artifact mean the discovery of a new diagnostic test, if less sensitive to short-term changes in blood pressure associated with veterinary care, would be useful. Identification of metabolomic changes in hypertensive cats could advance understanding of the pathogenesis of hypertension in cats, as well as identify novel biomarkers.
Use untargeted metabolomics to identify biochemical changes in cat plasma and urine between normotensive controls (NT) and hypertensive cats before treatment (HTpre); HTpre and hypertensive cats treated with amlodipine (HTtx).
Biobanked surplus plasma and urine samples were selected from client-owned cats (> 9 years old) that were NT (urine n = 17, plasma n = 19), HTpre (urine n = 13, plasma n = 19), or HTtx (urine n = 12, plasma n = 19).
Samples were profiled using flow infusion electrospray-high-resolution mass spectrometry, and differences assessed using univariate (paired or two sample t-tests) and multivariate (partial least squares discriminant analysis) methods using the R-based MetaboAnalyst platform. Tentative identifications of metabolites then were made using the MZedDb database.
Significant (false discovery adjusted < 0.01) biochemical differences were observed between each of the sample groups. Biochemical changes in urine between HTpre and NT animals were linked to the tricarboxylic acid cycle, oxidative stress, steroid hormones, taurine metabolism, and phosphatidylinositol-3,4,5-trisphosphate.
Metabolites altered in hypertensive cats were similar to those observed in other species.
猫高血压的早期诊断仍是一个重要问题。基层医疗实践中缺乏常规血压筛查，且存在白大衣假象，这意味着如果发现一种对与兽医护理相关的血压短期变化不太敏感的新诊断测试将很有用。识别高血压猫的代谢组学变化有助于深入了解猫高血压的发病机制，并识别新的生物标志物。
使用非靶向代谢组学来识别正常血压对照猫（NT）与治疗前高血压猫（HTpre）以及HTpre与接受氨氯地平治疗的高血压猫（HTtx）之间猫血浆和尿液中的生化变化。
从客户拥有的年龄大于9岁的猫中选取生物样本库中的多余血浆和尿液样本，这些猫分为NT组（尿液n = 17，血浆n = 19）、HTpre组（尿液n = 13，血浆n = 19）或HTtx组（尿液n = 12，血浆n = 19）。
使用流动注射电喷雾高分辨率质谱对样本进行分析，并使用基于R的MetaboAnalyst平台通过单变量（配对或两样本t检验）和多变量（偏最小二乘判别分析）方法评估差异。然后使用MZedDb数据库对代谢物进行初步鉴定。
在每个样本组之间均观察到显著（错误发现率调整后<0.01）的生化差异。HTpre组和NT组动物尿液中的生化变化与三羧酸循环、氧化应激、类固醇激素、牛磺酸代谢和磷脂酰肌醇-3,4,5-三磷酸有关。
高血压猫中改变的代谢物与其他物种中观察到的相似。
