Dietrichs E, Walberg F, Nordby T
Neurosci Res. 1985 Oct;3(1):52-70. doi: 10.1016/0168-0102(85)90038-0.
The implantation technique described by Mori et al. has been modified for the implantation of crystalline wheat germ agglutinin-horseradish peroxidase (WGA-HRP) complex. This method permits a detailed analysis of the afferent and efferent connections of the cerebellar nuclei without the complication of uptake and transport of the tracer into passing fibres. We have used this method for studies of the olivo-dentate and dentato-olivary projections in the cat. After implantation of WGA-HRP into the dentate nucleus in all our cases, both anterogradely labelled terminal dentato-olivary fibres and retrogradely labelled olivo-dentate neurons were found in the contralateral inferior olive. It appears from our findings that both projections are topically organized. The dorsal part of dentate nucleus is bidirectionally connected with the rostral part of the principal olive, the ventrolateral part of the dentate is connected with the intermediate portion of the principal olive, while its ventromedial part is connected with the caudal portion of the principal olive. The olivo-dentate and dentato-olivary connections appear to be largely reciprocally organized. The advantages and drawbacks encountered with implantation of crystalline WGA-HRP are discussed, and our observations are considered in relation to previous studies on the olivo-cerebellar and cerebello-olivary connections.
森田等人描述的植入技术已针对结晶小麦胚凝集素 - 辣根过氧化物酶（WGA - HRP）复合物的植入进行了改进。该方法允许对小脑核的传入和传出连接进行详细分析，而不会出现示踪剂被传入纤维摄取和运输的复杂情况。我们已将此方法用于猫的橄榄核 - 齿状核和齿状核 - 橄榄核投射的研究。在我们所有的病例中，将WGA - HRP植入齿状核后，在对侧下橄榄核中发现了顺行标记的终末齿状核 - 橄榄核纤维和逆行标记的橄榄核 - 齿状核神经元。从我们的研究结果来看，这两种投射似乎都是按局部组织的。齿状核的背侧部分与主橄榄核的 Rostral 部分双向连接，齿状核的腹外侧部分与主橄榄核的中间部分连接，而其腹内侧部分与主橄榄核的尾侧部分连接。橄榄核 - 齿状核和齿状核 - 橄榄核连接似乎在很大程度上是相互组织的。讨论了植入结晶WGA - HRP时遇到的优缺点，并结合先前关于橄榄核 - 小脑和小脑 - 橄榄核连接的研究对我们的观察结果进行了考虑。
