Webb Alexander J, Zhao Qin-Ping, Allan Fiona, Kelwick Richard J R, Emery Aidan M, Freemont Paul S
Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, London, UK.
Department of Parasitology, School of Basic Medical Sciences, Wuhan University, Wuhan, Hubei Province, People's Republic of China.
Commun Biol. 2025 Aug 30;8(1):1321. doi: 10.1038/s42003-025-08773-7.
The neglected tropical disease schistosomiasis continues to be a global health concern, especially in low- and middle-income countries, with at least 250 million people infected worldwide and a further 779 million at risk of infection. Schistosoma japonicum, which is found in parts of South Asia, causes intestinal schistosomiasis in humans, as well as infecting up to forty other mammalian species. Therefore, novel diagnostics that can detect S. japonicum are desirable. In this study, we have further developed and refined the 'SNAILS' DNA-based biosensor technology for the detection of schistosomes and have applied this to the specific detection of S. japonicum. Of the four new 'SNAILS' probe pairs developed, one of our optimised designs successfully detected and differentiated between genomic DNA isolated from 16 S. japonicum cercariae isolated from sites in the People's Republic of China and laboratory-derived Schistosoma mansoni cercariae.
被忽视的热带病血吸虫病仍然是一个全球卫生问题，特别是在低收入和中等收入国家，全球至少有2.5亿人感染，另有7.79亿人面临感染风险。日本血吸虫存在于南亚部分地区，可导致人类患肠道血吸虫病，还可感染多达四十种其他哺乳动物。因此，需要能够检测日本血吸虫的新型诊断方法。在本研究中，我们进一步开发和完善了用于检测血吸虫的基于DNA的“SNAILS”生物传感器技术，并将其应用于日本血吸虫的特异性检测。在新开发的四对“SNAILS”探针中，我们优化设计的其中一对成功检测并区分了从中华人民共和国各地点分离出的16只日本血吸虫尾蚴以及实验室培育的曼氏血吸虫尾蚴的基因组DNA。
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