锌基传统药物雅沙德·巴斯马的特性及潜在新应用

Characterization and potential novel applications of zinc-based traditional medicine, Yashad Bhasma.

作者信息

Nille Guruprasad C, Bhuyan Monisha, Gupta Laxmi Narayan, Ali Mohd, Tripathi Chandra Shekhar Pati, Nille Omkar S, Mishra Shardendu Kumar, Vibhute Anuja, Kamble Pranoti, Ranjan Himanshu, Singh Amaresh Kumar, P-Tiwari Arpita, Chaudhary Anand Kumar

机构信息

Department of Rasa Shastra and Bhaishajya Kalpana, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, U.P., India.

Ayurvedic Medical Officer, AHWC (GAD), Humma Ganjam, Odisha, 761027, India.

出版信息

J Ayurveda Integr Med. 2025 Aug 30;16(5):101188. doi: 10.1016/j.jaim.2025.101188.

DOI:10.1016/j.jaim.2025.101188

PMID:40886520

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12419013/

Abstract

BACKGROUND

Yashad Bhasma (YB), the incinerated metal ash of zinc, has been used for centuries in Ayurveda to address a variety of conditions, including eye diseases, diabetes mellitus, anemia, respiratory illnesses, etc. OBJECTIVE: This research aimed to synthesize and characterize YB and to evaluate its potential antimicrobial, antioxidant, and anti-angiogenic activities.

MATERIALS AND METHODS

In this study, YB is synthesized by optimizing the traditional method. Morphological and physicochemical characterization are performed using XRD, XPS, SEM, TEM, EDAX, DLS, TGA-DSC, and FTIR. The antimicrobial activity of YB is assessed using the well diffusion technique against the gram-positive bacterium Staphylococcus aureus (S. aureus) and the gram-negative bacterium Escherichia coli (E. coli). The antioxidant potential is evaluated using the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging assay and Nitric oxide (NO) radical scavenging assay. A chick chorioallantoic membrane (CAM) assay is performed on fertilized chick eggs to study the anti-angiogenesis potential of YB.

RESULTS

The XRD patterns of YB showed the presence of cubic and hexagonal phases of ZnS having average crystallite size of 32.66 nm. XPS data supports the formation of ZnS phase of YB. SEM and TEM data confirmed the size of YB NPs in a range of 250-350 nm. The EDAX analysis confirmed the presence of Zn (37.2 %) and S (21.18 %). The mean particle diameter was 361 nm in DLS. TGA-DSC findings verified that the synthesized material is stable up to 435.80 °C. The FTIR confirms the presence of organic moieties in YB along with ZnS phase. YB effectively inhibited the growth of S. aureus and E. coli. The ability of YB to scavenge DPPH and NO radicals is found to be concentration dependent (50-250 μg/mL). The study also demonstrated that YB has notable antioxidant activity. The disappearance of blood vessels beneath the sample-loaded disk after 7 days indicated the effective anti-angiogenic properties of YB.

CONCLUSION

Altogether, YB exhibited significant antimicrobial, noteworthy antioxidant, and anti-angiogenic activities, indicating its potential as a promising therapeutic agent.

摘要

背景

雅沙德药灰（YB）是锌的煅烧金属灰，在阿育吠陀医学中已使用数百年，用于治疗多种病症，包括眼部疾病、糖尿病、贫血、呼吸系统疾病等。目的：本研究旨在合成并表征YB，并评估其潜在的抗菌、抗氧化和抗血管生成活性。

材料与方法

在本研究中，通过优化传统方法合成YB。使用XRD、XPS、SEM、TEM、EDAX、DLS、TGA-DSC和FTIR进行形态学和物理化学表征。采用打孔扩散法评估YB对革兰氏阳性菌金黄色葡萄球菌（S. aureus）和革兰氏阴性菌大肠杆菌（E. coli）的抗菌活性。使用1,1-二苯基-2-苦基肼（DPPH）自由基清除试验和一氧化氮（NO）自由基清除试验评估抗氧化潜力。在受精鸡蛋上进行鸡胚绒毛尿囊膜（CAM）试验，以研究YB的抗血管生成潜力。

结果

YB的XRD图谱显示存在立方相和六方相的ZnS，平均微晶尺寸为32.66 nm。XPS数据支持YB的ZnS相形成。SEM和TEM数据证实YB纳米颗粒的尺寸在250 - 350 nm范围内。EDAX分析证实存在Zn（37.2%）和S（21.18%）。DLS测定的平均粒径为361 nm。TGA-DSC结果证实合成材料在高达435.80 °C时稳定。FTIR证实YB中除了ZnS相外还存在有机部分。YB有效抑制了金黄色葡萄球菌和大肠杆菌的生长。发现YB清除DPPH和NO自由基的能力呈浓度依赖性（50 - 250 μg/mL）。该研究还表明YB具有显著的抗氧化活性。7天后加载样品圆盘下方血管消失表明YB具有有效的抗血管生成特性。