Department of Medical Biotechnology, Center for Interdisciplinary Research, D.Y. Patil Education Society (Institution Deemed to be University), Kolhapur, Maharashtra, India.
Department of Medical Physics, Center for Interdisciplinary Research, D.Y. Patil Education Society (Institution Deemed to be University), Kolhapur, Maharashtra, India.
Lett Appl Microbiol. 2022 Oct;75(4):913-923. doi: 10.1111/lam.13764. Epub 2022 Jun 27.
Widespread and irrational use of antibiotics results in the development of antibiotic-resistant bacteria. Thus, there is a need to develop novel antibacterial agents in order to replace conventional antibiotics and to increase the efficacy of already existing antibiotics by combining them with other materials. Herein, a single-step antibiotic-mediated synthesis of antibiotic-conjugated gold nanoparticles is reported. In this single-step method antibiotic Kanamycin, an aminoglycoside itself plays the role of reducing as well as capping agent by reducing gold salt into gold nanoparticles. The kanamycin-conjugated gold nanoparticles (Kan-AuNPs) were confirmed by UV-Visible spectroscopy and further physico-chemically characterized by various instrumental techniques. Synthesized Kan-AuNPs showed broad-spectrum antibacterial activity against Gram-positive Staphylococcus aureus as well as Gram-negative Escherichia coli bacterial strains. They are also found to be effective against Pseudomonas aeruginosa and pathogenic E. coli isolated from urinary tract infections (UTIs) patients, which are responsible to cause hospital-acquired infections like nosocomial, burn wound and UTIs. The minimum inhibitory concentration (MIC) of Kan-AuNPs is 50 μg ml for S. aureus and E. coli, 125 μg ml for P. aeruginosa and 100 μg ml for E. coli isolated from UTIs patients. It is also evident that the MIC of Kan-AuNPs for antibacterial activity is lower as compared to antibiotic kanamycin alone for all bacterial strains. Hence, the one-step strategy of synthesis for Kan-AuNPs is a suitable strategy for fighting infectious bacterial strains in hospitals, healthcare and the pharmaceutical industry.
抗生素的广泛和不合理使用导致了抗生素耐药菌的产生。因此，需要开发新型抗菌剂来替代传统抗生素，并通过与其他材料结合来提高现有抗生素的疗效。在此，报道了一种通过抗生素介导的一步法合成抗生素偶联金纳米粒子的方法。在这种一步法中，抗生素卡那霉素本身作为氨基糖苷类，既作为还原剂，也作为封端剂，将金盐还原为金纳米粒子。通过紫外-可见光谱证实了卡那霉素偶联的金纳米粒子（Kan-AuNPs）的存在，并通过各种仪器技术进一步对其进行了物理化学特性的表征。合成的 Kan-AuNPs 对革兰氏阳性的金黄色葡萄球菌和革兰氏阴性的大肠杆菌表现出广谱的抗菌活性。此外，它们还对铜绿假单胞菌和从尿路感染（UTI）患者中分离出的致病性大肠杆菌具有抗菌活性，这些细菌会引起医院获得性感染，如医院内感染、烧伤创面感染和 UTI。Kan-AuNPs 的最小抑菌浓度（MIC）对金黄色葡萄球菌和大肠杆菌为 50μg/ml，对铜绿假单胞菌为 125μg/ml，对从 UTI 患者中分离出的大肠杆菌为 100μg/ml。Kan-AuNPs 对所有细菌的抗菌活性的 MIC 均明显低于单独使用抗生素卡那霉素。因此，Kan-AuNPs 的一步法合成策略是在医院、医疗保健和制药行业中对抗感染性细菌菌株的一种合适策略。
