Chen Liping, Wang Jiawei, Li Kangyuan, Chen Danni, Wang Qihao, Liu Jiamin, Kuang Jingwen, Cao Yong, Xiao Suyao
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University.
J Oleo Sci. 2025;74(9):805-818. doi: 10.5650/jos.ess25025.
1,3-dilinoleoyl-2-palmitoylglycerol (LPL) is an important structural lipid in breast milk fat, which plays an important role in the health of infants, and therefore the development of an efficient method for the preparation of such compounds is necessary. In the present study, LPL was efficiently catalytically synthesized by immobilized lipase ANL-MARE as a biocatalyst using tripalmitate and linoleic acid in a solvent-free system, and its digestive properties were investigated. The optimal process conditions for the enzymatic acidolysis of LPL were optimized by response surface test: the molar ratio of PPP:LA was 1:10, the enzyme addition was 13.60%, the reaction temperature was 50℃, and the reaction time was 5 h. At this time, the relative content of LPL in the product was 67.78%, of which the relative content of sn-2 palmitic acid (sn-2 PA) accounted for 71.50%. In vitro gastrointestinal digestion of LPL resulted in the release of 59.69% of its fatty acids. The digested product contained higher levels of free unsaturated fatty acids and palmitic acid monoacylglycerols. In conclusion, the immobilized enzyme ANL-MARE has great potential to catalyze the preparation of LPL, which provides a new strategy and theoretical basis for the efficient preparation of human milk fat substitutes.
1,3-二亚油酰基-2-棕榈酰甘油（LPL）是母乳脂肪中的一种重要结构脂质，对婴儿健康起着重要作用，因此开发一种高效制备此类化合物的方法很有必要。在本研究中，以固定化脂肪酶ANL-MARE作为生物催化剂，在无溶剂体系中利用三棕榈酸甘油酯和亚油酸高效催化合成LPL，并对其消化特性进行了研究。通过响应面试验优化了LPL酶促酸解的最佳工艺条件：PPP:LA的摩尔比为1:10，酶添加量为13.60%，反应温度为50℃，反应时间为5 h。此时，产物中LPL的相对含量为67.78%，其中sn-2棕榈酸（sn-2 PA）的相对含量占71.50%。LPL的体外胃肠道消化导致其59.69%的脂肪酸释放。消化产物中游离不饱和脂肪酸和棕榈酸单酰甘油的含量较高。综上所述，固定化酶ANL-MARE在催化制备LPL方面具有巨大潜力，为高效制备人乳脂肪替代品提供了新的策略和理论依据。
