Handler J S, Moran A
Pflugers Arch. 1985;405 Suppl 1:S163-6. doi: 10.1007/BF00581800.
A variety of techniques have been used to study the sodium-coupled hexose transporter in epithelia formed by LLC-PK1 cells. The expression of the transporter is affected by the density and age of the culture and by the concentration of glucose in the growth medium. Sodium-coupled hexose transport appears as the epithelium becomes confluent and increases further as the epithelium matures. The increased transport is associated with increased transport in apical plasma membrane vesicles. Epithelia grown in medium containing 5 mM glucose express more transporters than epithelia grown in medium containing 25 mM glucose. The increase in transport is not the result of an extracellular signal that is generated as a consequence of the concentration of glucose. The response to different hexoses that are or are not transported on the carrier indicates that it is the metabolism of glucose that acts as the signal for expression of more or fewer transporters. The results are compared to similar studies of the effects of substrate concentration on expression of transporters in cultured fibroblasts and the intestines in situ.
已经使用了多种技术来研究LLC-PK1细胞形成的上皮细胞中钠偶联己糖转运体。转运体的表达受培养物的密度和年龄以及生长培养基中葡萄糖浓度的影响。随着上皮细胞汇合，钠偶联己糖转运出现，并且随着上皮细胞成熟进一步增加。转运增加与顶端质膜囊泡中的转运增加有关。在含有5 mM葡萄糖的培养基中生长的上皮细胞比在含有25 mM葡萄糖的培养基中生长的上皮细胞表达更多的转运体。转运的增加不是由于葡萄糖浓度产生的细胞外信号的结果。对载体上是否转运的不同己糖的反应表明，是葡萄糖的代谢作为表达更多或更少转运体的信号。将这些结果与关于底物浓度对培养的成纤维细胞和原位肠道中转运体表达影响的类似研究进行了比较。
